Research Associate - Genome Editing

Location
San Francisco, CA, United States
Posted
Sep 04, 2020
Ref
953695443
Hotbed
Biotech Bay
Required Education
Associate Degree
Position Type
Full time
Mammoth Biosciences is harnessing the diversity of nature to power the next-generation of CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. By leveraging its internal research and development and exclusive licensing to patents related to Cas12, Cas13, Cas14 and CasPhi, Mammoth Biosciences can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Mammoth is democratizing disease detection with easy and affordable point-of-care tests that allow real-time and simultaneous detection of multiple conditions, along with high-throughput tests that allow for unprecedented testing volume. Further, the company is transforming disease treatment with its proprietary micro-sized CRISPR proteins that enable new editing and delivery options. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Decheng, Mayfield, NFX, and 8VC, and leading individual investors including Brook Byers, Tim Cook, Bob Nelsen, and Jeff Huber. At Mammoth, we are passionate about transforming lives through innovation.

DESCRIPTION

Mammoth is currently seeking a talented and detail-oriented Research Associate with experience in molecular biology, flow cytometry, mammalian cell culture, and genetic manipulation of immune cells and/or iPSCs, to join our growing genome editing team. The successful candidate will work closely and collaboratively with colleagues to design/develop, perform and execute experiments on mammalian cell lines and primary human cells to support pipeline projects to develop Cas nucleases for genome editing. They will have the opportunity to contribute broadly across multiple technical areas. The position requires adaptability, excellent planning, strong organizational and communication skills, as well as the ability to work independently in a fast-paced team-oriented and dynamic environment.

Responsibilities
    • Execution of molecular biology, cloning, cell culture and a broad range of functional and phenotypic assay workflows to accelerate projects in pre-clinical development.
    • Provide team support for basic laboratory tasks including tissue culture of various cell lines, isolation and culture of primary human cells from whole blood, phenotyping and analytical characterization of cells, genome editing of primary human cells, and virus production
    • Accurately and timely record, document, analyze and communicate laboratory data to senior staff and team members
    • Conducts all activities in a safe and efficient manner, and adheres to site environmental health and safety (EHS) requirements


Requirements
    • BS or MS or in molecular and cell biology, Immunology, biochemistry, or a related field, plus at least 3 years relevant laboratory research experience. Industry work experience, especially in immunology/immuno-oncology field, is highly desirable.
    • Proficiency in mammalian cell culture, isolation and culture and genetic manipulation of primary human immune cells.Previous work experience with human NK and T cells and/or CAR-T cell production and characterization is highly desirable.
    • Proficiency with multi-color flow cytometry, FACS sorting and T-cell based assays (T cell phenotyping and memory sub-setting, cytotoxicity, cell proliferation, cytokine secretion).
    • Proficiency in molecular cloning, PCR, qPCR, genomic DNA and mRNA purification, NGS sequencing, ELISA, western blot, retroviral/lentiviral/AAV production, titration, and transduction.
    • Strong organizational and record-keeping skills, excellent interpersonal, verbal and written communication skills. Highly motivated with strong work ethics. Ability to work efficiently and productively in a fast-paced highly collaborative team environment
    • Knowledge of Microsoft Office Suite and other relevant data analysis software (Flowjo, GraphPad Prism, etc.).


Benefits
    • Competitive salary
    • Full health/vision/dental benefits
    • Unlimited vacation
    • Free breakfast, lunch, dinner, and barista
    • Free gym
    • Public transportation commuter subsidy
    • 401(k) matching