Senior Research Associate, Gene Therapy

JOB PURPOSE

Sana Biotechnology is seeking a creative and highly-motivated individual to join our Gene Therapy - Vector Design team. This individual will be responsible for advancing viral vector pipelines by generating and testing crucial research material in in vitro assays and performing IND-enabling preclinical experiments, while working closely with other members of the gene therapy and process development teams.

DUTIES AND RESPONSIBILITIES

• Planning, designing and executing complex experiments.
• Working with complex primary cells and analyzing them via multi-color flow cytometry.
• Using molecular biology tools to assess the performance of new vector designs.
• Building genetic libraries of variants, using high-throughput molecular cloning methods.
• Optimization of viral vector production protocols, including staying up-to-date with current knowledge while driving innovation.
• Working closely with other members of the gene therapy and process development teams to share and apply knowledge advancements.
• Reviewing and presenting data cross-functionally at department meetings and larger forums.
• Comply with standard laboratory practices and company policies, including strict notebook documentation and biosafety policies.
• Contribute to the overall Sana mission and culture.

QUALIFICATIONS

Basic Qualifications

• BS in scientific field plus 4+ years of experience, or M.S., 1-2 yrs in biotechnology, molecular biology, biochemistry, protein chemistry, virology or related discipline with 3 years of relevant industry laboratory experience, with a focus towards gene therapy and high-throughput assays.
• Demonstrated expertise in mammalian cell line and primary cell cultures, and lentiviral vector production.
• Experience in molecular biology, including PCR, RT-qPCR, restriction digest/ligation, Gibson/Golden Gate assembly, plasmid prep.
• Independently and proactively able to design and execute experiments including interpreting and presenting data.
• Excellent bench skills with attention to detail and high standards for data integrity and quality.
• Adherence to rigorous standards of good lab practices, safety, and documentation.
• Ability to work as part of a multi-site team.

Preferred Qualifications

• Experience with liquid handler automation platforms (e.g. Integra, Hamilton, Tecan)
• Experience modifying cellular genomes with CRISPR-Cas9 gene editing
• Knowledge of multi-color flow cytometry principles and flow cytometry data analysis

WORKING CONDITIONS

This is a laboratory bench role. No regular travel required.