Research Associate

Location
San Francisco, CA, United States
Posted
Aug 19, 2020
Ref
REQ-485
Discipline
Science/R&D, Research
Hotbed
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time
Category:
Science
Lab/Area:

Marson Lab

Description:

We are recruiting a Research Associate to assist with ongoing CRISPR-Cas9 pooled gene editing projects focussed on developing a deeper understanding of Human Immunodeficiency Virus (HIV) biology. Our lab has pioneered the application of novel genetic engineering technologies such as CRISPR to edit primary human immune cells (Schumann et al. PNAS 2015). We have successfully used this technology to study the functional role of host genes in HIV infection and replication (Hultquist et al. Cell Reports 2016; Hultquist and Hiatt et al. Nat Biotechnol 2019). We can now perform genome-wide high throughput loss-of-function screens in primary T-cells (Shifruit and Carnevale et al. Cell 2018). The ability to precisely replace DNA sequences in the genome of functional T cells in a pooled format provides unprecedented opportunities to test the mechanistic effects of all host and viral genetic factors in viral pathogenesis in an unbiased manner. In addition to cell editing, our lab integrates genomic analysis (e.g. ChIP-seq, ATAC-seq, RNA-seq), human disease genetics, and high dimensional screening approaches to develop a better understanding of our immune system (Simeonov et al. Nature 2017; Shifrut and Carnevale et al. Cell 2018). We are now building on these innovations by developing new tools for drug target discovery and employing them alongside our existing platform to identify novel regulators of HIV latency. This work also has the potential to be widely applicable to various other human pathogenic viruses.

Working as part of the infectious biology group in the Marson lab, this position will utilize various protocols to generate the molecular components, such as DNA constructs, necessary for specific cellular engineering experiments. Additional major responsibilities will include the preparation, sequencing, and analysis of next-generation sequencing libraries to support pooled screening experiments. Further responsibilities will consist of isolation and culture of primary human immune cells, as well as functional analysis of engineered cells via Sanger sequencing and flow cytometry. The projects will also involve purification of infectious HIV virus and infection of T cells with HIV in a biosafety level-3 facility. Strong organizational and communication skills are required. For exceptionally skilled applicants, opportunity exists to extend the role to include the design and completion of cellular editing experiments, as well as in vivo analysis of novel host-virus interactions. This position represents an excellent opportunity to gain exposure to basic laboratory skills as well as genetics, genome engineering and virology skills in a dynamic, well-established research environment.

Additional Information:

• Title and salary will be commensurate with education and experience.

• Be a part of big ideas and consider Gladstone. Your difference will make a difference in human health.

• Gladstone is an equal opportunity employer

Required Qualifications:

• BA/BS degree and one or more years of laboratory experience utilizing techniques or methods required by the position; or an equivalent combination of education and experience

• Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.)

• Excellent organizational and interpersonal communication skills (verbal and written)

• Willingness and ability to learn new methods and skills for changing research priorities

• Willingness to work with infectious HIV virus in a BSL-3 facility

• Ability to work independently and as a member of a research team

• Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines

Preferred Qualifications:

• Prior experience with DNA sequencing technologies, including Sanger and NGS

• Prior experience with next-generation sequencing library preparations and quality control

• Prior experience in the design and assembly of DNA constructs (via restriction digestion and ligation, Gibson assemblies, Golden-Gate assemblies, etc.)

Additional Information:

  • Title and salary will be commensurate with education and experience.
  • Be a part of big ideas and consider Gladstone. Your difference will make a difference in human health.
  • Gladstone is an equal opportunity employer.