Senior Research Associate, Assay and Bioanalytical Development

Location
South San Francisco, California, United States
Posted
Apr 06, 2020
Ref
4643358002
Hotbed
Biotech Bay
Required Education
Bachelors Degree
Position Type
Full time
Company Description:

Ambys Medicines is a biotechnology company focused on discovering and developing regenerative and restorative therapies for people with advanced liver disease. Founded by world-renowned experts in liver disease and regenerative medicine, Ambys is pioneering the application of novel modalities including cell and gene therapy, and gain-of-function drug therapy, to meet the urgent need for treatments that have the potential to restore liver function and prevent the progression to liver failure across multiple liver diseases that are untreatable or poorly treated today. Ambys was launched in 2018 by Third Rock Ventures and Takeda Pharmaceuticals with $140M in starting capital and is headquartered in South San Francisco, California.

Position Summary:

We are seeking an enthusiastic and dynamic Senior Research Associate to make impactful contributions to the Assay and Bioanalytical Development group. Our team is responsible for developing characterization assays to support the Cell Therapy Program as well as analytical assays to support AAV Gene Therapy products. In this position, you will primarily be responsible for designing and developing flow cytometry-based assays. In addition, due to the sheer number of assays requiring development, you will have a strong opportunity to broaden your technical acumen beyond flow cytometry. You will interface with multidisciplinary groups so must not only be able to work independently but collaboratively in a fast-paced interactive environment.

What you will do:
  • Strategically design and execute fundamentally sound flow cytometry experiments with minimal supervision.
  • Self-motivated to independently optimize, troubleshoot and improve assays.
  • Develop assays with goal of drafting protocols for transfer to manufacturing.
  • Maintain thorough and well-organized experimental records.
  • Collect, analyze and clearly present findings to peer scientists in research meetings

Qualifications:
  • BS/BA or MS in a biological science discipline, 6+ years of experience in industry or academia
  • Technically proficient to independently design and optimize multi-parameter flow cytometry assays. Experience dealing with challenging mixed-species samples derived from humanized rodent animals is a plus.
  • Strong cell culture skills with fundamental understanding of developing cell-based assay using primary cells and immortalized cell lines. Experience with primary human hepatocytes is preferred.
  • Experience with in vitro AAV transduction and non-viral transfection.
  • Competent in diverse molecular and cellular biology techniques, including nucleic acid extraction, RT-qPCR, ddPCR, Western Blot, Luciferase Reporter Assays, ELISA, AlphaLISA, Multiplex Assays (Luminex)
  • Experience with NGS and RNA-Seq is a plus.
  • Strong ability to interpret and communicate experimental results
  • Familiarity with data analysis software, including Microsoft Excel, GraphPad Prism, FlowJo, JMP, etc.
  • Research background in liver biology, gene therapy, or cell therapy is a plus