Scientist – Plasmid Production and Purification
- Employer
- Homology Medicines
- Location
- Bedford, MA
- Start date
- Nov 15, 2019
View more
- Discipline
- Science/R&D, Bio-Assay Development, Biology, Chemistry, Molecular Biology
- Required Education
- Masters Degree/MBA
- Position Type
- Full time
Job Details
Scientist – Plasmid Production and Purification
Location: Bedford, MA
Homology Medicines seeks an outstanding Scientist in the Upstream Process Development team to oversee the development of plasmid DNA product manufacturing platforms. The individual in this role will be a highly-skilled, talented and motivated scientist who will focus on the development, characterization, and scale up of methods for producing plasmid DNA. We seek candidates with proven ability to work independently after initial training and collaboratively to achieve optimized project plans, with the goal to deliver products that transform the lives of patients.
Essential Functions and Duties
- Oversee optimization of critical scientific and engineering parameters for DNA production including bacterial cell culture and fermentation, harvest, feed strategies, and lysis and clarification
- Purification experience with chromatographic purification or HPLC evaluation using IEX or HIC
- Evaluate raw materials and equipment ensuring scalability and compatibility with GMP operations
- Molecular techniques including DNA sequencing, restriction digest analysis, gel electrophoresis, and ELISAs
- Develop SOP’s, batch records, and provide technical support and training to others
- Ability to support regulatory documentation from needed for submissions
Skills and Experience
- BS (6+), MS (3+), or PhD (1+) years industry experience. Degree experience in biochemistry, chemistry, or molecular biology
- Previous production of biologic products in bacterial culture systems
- Industry experience in the manufacturing of DNA products in fermentation bioreactors
- Purification experience with anion exchange or hydrophobic interaction chromatography
- Able to effectively manage and prioritize multiple parallel laboratory activities is essential
- Excellent interpersonal written and oral communication skills and ability to work as part of multidisciplinary teams
Company
Homology Medicines is based on groundbreaking science that harnesses the naturally occurring process of homologous recombination. This non-nuclease-based approach offers clear advantages in its precision, efficiency and on-target in vivo editing of genetic mutations. Homology obtained an exclusive worldwide license to this technology platform, which is based on the pioneering research of Saswati Chatterjee, Ph.D., Professor of Virology at the Beckman Research Institute at the City of Hope in California, member of the Recombinant DNA Advisory Committee (RAC) to the Office of the Director, National Institutes of Health (NIH) and former charter member of the Therapeutic Approaches to Genetic Diseases Study Section of the NIH. Dr. Chatterjee and her team led the first adeno-associated virus (AAV) vector-mediated gene transfer studies into human hematopoietic stem cells and subsequently identified and isolated a series of naturally-occurring AAVs from human CD34+ cells.
YEAR FOUNDED:
2015
LEADERSHIP:
Founder: Saswati Chatterjee
President and CEO: Arthur Tzianabos, Ph.D.
CSO: Albert Seymour, Ph.D.
- Website
- http://www.homologymedicines.com/
- Phone
- 857-305-1825
- Location
-
1 Patriot Place
Bedford
Massachusetts
01730
US
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