Post Doctoral Fellow

Location
Frederick, MD, USA
Posted
Sep 11, 2019
Ref
req351
Hotbed
BioCapital
Required Education
Doctorate/PHD/MD
Position Type
Full time
LHRI - The Laboratory of Human Retrovirology and Immunoinformatics (LHRI), Clinical Services Program, Applied/ Developmental Directorate, has investigated HIV pathogenesis and innate immune response against HIV or HIV-related virus infection. LHRI has several single nucleotide polymorphisms (SNPs) in HIV genome from patients infected with multiple drug resistant (MDR) HIV strains. In this position, a candidate will investigate and characterize the impact of the SNPs in drug sensitivity and viral fitness collaborating with National Institute of Allergy and Infectious Diseases (NIAID).

KEY ROLES/RESPONSIBILITIES
  • Development of a novel gene therapies to suppress MDR-HIV or other virus infection utilizing a combination of CRISPR/Case9 (or other gene editing methods) and other protocols

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
  • Possession of a PhD or MD/PhD degree from an accredited college or university according to the Council for Higher Education Accreditation. Foreign degrees must be evaluated for U.S. equivalency
  • No experience beyond the Doctorate degree required
  • Must be able to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:
  • General molecular biology techniques, construction of expression plasmids
    • Tissue culture (cell lines and primary human cells)
    • DNA transfection to primary cells using 4D system
    • PCR, qRT-PCR
    • Western blotting
    • Immune precipitation assay
    • Protein isolation using FPLC
    • Imaging analysis of a Confocal or fluorescence microscopy
    • CRISPR technology
    • Handling of replication competent HIV virus and lentivirus
    • AMNIS

EXPECTED COMPETENCIES
  • Strong background in immunology and knowledge in gene editing, CRISPR or other techniques
  • The ability to challenge and establish new assay system(s)


Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)