R&D Specialist, Viral Vector Technology

Location
Tarrytown, NY, United States
Posted
Sep 07, 2019
Ref
17466BR
Required Education
Masters Degree/MBA
Position Type
Full time
Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

Summary:

Regeneron is currently seeking a talented and motivated Research Associate to join a technology development group which aims to develop novel viral-based vectors. The successful candidate should have a strong background in molecular biology, virology, and cell biology. Incumbent should be able to work methodically and with minimal supervision. His/her main goal will be to develop novel viral vector-mediated gene therapy approaches for cancer/blood disease/hematological disorders. Main tasks will include production, purification and characterization of novel viral vectors in various in vitro and in vivo assays. Experience with immunological assays and virus production is desired.

Specific experience should include mammalian cell culture, basic molecular biology, protein expression assays, immunological assays, manipulation of viruses and recombinant proteins in BSL2 conditions.

Responsibilities:

1. Maintain, transfect, and transduce mammalian cell cultures

2. Design pseudotyped viral vectors

3. Produce, concentrate, purify, quantify, and characterize viral vectors

4. Evaluate in vitro virus transduction and transgene expression in various cell types, including hematopoietic cells (T cells, B cells, Hematopoietic stem cells, dendritic cells, macrophages)

5. Perform basic biochemical/immunological assays (ELISA, flow cytometry)

6. Perform basic molecular biology assays (Cloning, Western blotting, DNA/RNA extractions, PCR, RT-PCR, quantitative PCR, etc.)

7. Perform in vivo injections of viral vectors in animal models and evaluate the subsequent phenotypes using different assays (live imaging, immune-histochemistry, FACS,...)

8. Manipulate viral vectors in BSL2 cell culture and animal facilities

9. Analyze and present data

10. Maintain lab supplies and help with lab organization

Requirements:

Master's Degree and minimum 3 years of research experience.

TECHNICAL SKILLS:
Cell culture, immunology, virology, basic molecular biology/biochemistry, flow cytometry, ELISA/MSD, experience with animals (general handling, injections of pharmacological compounds, tissue processing), experience with imaging

CORE COMPETENCIES:
- Incumbent should be highly skilled in molecular biology / virology / immunology and have sufficient practical experience to be able to carry out well-controlled experiments that can provide reliable results with minimal supervision
- Incumbent should be able to analyze data and extract conclusions
- Incumbent should have experience with basic safety procedures and sterile techniques required for BSL2 laboratory function
- A general understanding of microbiology and general immunology is preferred
- Understanding of and experience with in vitro and in vivo assays with viral vectors will be a distinct advantage
- Some experience with virus purification is strongly desired

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.
JNH