Scientist I, Translational Research in Imaging

Location
Frederick, MD, USA
Posted
Aug 24, 2019
Ref
req704
Hotbed
BioCapital
Required Education
Doctorate/PHD/MD
Position Type
Full time
The Clinical PD Biomarkers Program is responsible for identification of biomarkers of response to new targeted agents entering clinical trials in the Developmental Therapeutics Program of the National Cancer Institute. The scope of work includes assay design, development, validation, fitness for purpose testing, transfer of assays to clinical laboratories, and clinical specimen analysis when required. The program facilitates mechanistic and performance analysis of putative anti-cancer agents, diagnostic tests, and therapeutic interventions to clinical applications, including distinct molecular signatures for cancer, refined molecular assays, and state-of-the-art imaging techniques that are too high-risk for industry or academia to pursue. The program operates several advanced, specialized instrumentation for image analysis of tumor biopsies, circulating tumor cells, and activated Tcells for measuring drug effects in patients enrolled in clinical trials.

KEY ROLES/RESPONSIBILITIES-THIS POSITION IS CONTINGENT UPON FUNDING APPROVAL
  • Develop and validate imaging approaches for multiplex immunofluorescence assays on state-of-the-art whole slide imaging platform in support of clinical studies and initiatives
  • Responsible for mastering novel imaging technology, software and instrumentation
  • Management of large data sets generated from image acquisition platform
  • Data analysis of immunofluorescence assay studies on fixed xenograft tissue sections and clinical specimen biopsies using advanced quantitative image analysis software
  • Design, test and write detailed technical SOPs for imaging and analysis for transfer to external labs
  • Generate materials and data necessary to support the primary experimental objectives of the program
  • Design, perform, analyze, and present in vitro experiments to test pharmacodynamic marker responses to novel anti-cancer therapeutics in multiple formats, but particularly in multiplex immunofluorescence analysis
  • Support development and implementation of multiplex immunofluorescence assays in support of clinical studies and initiatives on flow cytometry and microscopy platforms
  • Design experiments and strategies to test and confirm putative mechanism of action of clinical trial candidate compounds
  • Perform laboratory analysis, data analysis and reporting of results from specimens obtained from patients enrolled in clinical trials to the NCI

BASIC QUALIFICATIONS
  • Possession of a Doctoral degree from an accredited university according to the Council for Higher Education Accreditation. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency
  • No experience beyond the doctorate is required
  • Demonstration of specific training in cancer biology, biomarker development, or drug screening strategies is required
  • Experience in the design and validation of multiplexed immunofluorescence assays and the ability to work across testing platforms during the assay development process
  • Experience with quantitative image analysis software
  • Experience with flow cytometry, imaging flow cytometry
  • Design of in vitro, cell-based assays to test mechanism of action of candidate compounds
  • Ability to master novel technologies and instrumentation in response to changes in project priorities or breakthrough technological innovations; hands-on instrumentation work is required for this position
  • Ability to communicate with program lead researchers in a demanding scientific environment through preparation of formal reports is essential
  • pharmacology; cancer biology; flow cytometry; high content image analysis; immunofluorescence microscopy
  • Ability to maintain precise records
  • Must be able to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS
  • Experience with advanced imaging instrumentation, software and technologies
  • Experience with tissue-based immunofluorescence assays
  • Statistical analyses approaches
  • Data management
  • Experience in isolation and characterization of individual target cells
  • Experience with circulating tumor cell or T cell subset analysis
  • A record of publication in refereed scientific literature
  • Completion of related post-doctoral research and/or training

JOB HAZARDS OR REQUIREMENTS
  • Candidate will be working with or have the potential for exposure to animal(s) and/or animal material(s)
  • Candidate will be working with or have the potential for exposure to infectious material(s) or Other Potentially Infectious Materials (OPIM), requiring medical clearance and immunization in accordance with protocol requirements
Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)