Fermentation Associate
- Employer
- Frederick National Laboratory for Cancer Research
- Location
- Frederick, MD
- Start date
- Aug 12, 2019
View more
- Discipline
- Science/R&D, Biology, Cell Biology, Molecular Biology
- Required Education
- Bachelors Degree
- Position Type
- Full time
- Hotbed
- BioCapital
Job Details
KEY ROLES/RESPONSIBILITIES-THIS POSITION IS CONTINGENT UPON FUNDING APPROVAL
The Protein Expression Laboratory (PEL) provides support to the NCI RAS Initiative through generation and qualification of protein reagents for biochemistry, biophysics, structural biology, and drug screening projects. The PEL creates expression constructs, expresses the encoded recombinant proteins in multiple expression systems, and purifies the recombinant proteins for use in downstream applications. The Fermentation Associate will:
- Carry out experiments, under the review of a scientist, in the areas of prokaryotic and eukaryotic protein expression
- Carry out bacterial fermentation and eukaryotic cell culture
- Maintain scientific equipment in good working order
- Perform microscale protein purification scouting
- Perform large-scale purification using FPLC technology
- Carry out QC on proteins to ensure high quality reagent production
- Provide timely updates of project progress to supervisor and other staff in both informal and formal reports
- Maintain detailed records of all laboratory processes and procedures for quality assurance purposes
BASIC QUALIFICATIONS
- Possession of a Bachelor's degree from an accredited college/university according to the Council for Higher Education Accreditation (CHEA) in a related field (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency.
- In addition to educational requirements, a minimum of two (2) years progressively responsible job-related biomedical experience
- Knowledge of or experience with growing and maintaining pure cultures of microorganisms
- Must be able to obtain and maintain a security clearance
PREFERRED QUALIFICATIONS
- Experience with aseptic technique for prokaryotic or eukaryotic cell culture
- Ability to handle multiple scientific projects with a high degree of accuracy
- Experience with E. coli fermentation at scales up to 60 liters
- Experience with lysis methods for gram-negative bacteria
- Experience with FPLC-based protein purification
- Experience in microscale protein purification
- Experience with affinity-based methods of recombinant protein purification specifically IMAC
- Experience with standard chromatographic modalities of IEX and SEC in the context of FPLC-based column chromatography
Company
A rewarding career with global impact
Whether you’re an expert in your field or just starting out, we have a career opportunity for you. We’re always looking for people to join us in fulfilling the mission of the Frederick National Laboratory: discovery, innovation, and success in the biomedical sciences.
Our team of 2,400+ scientists, technicians, administrators, and support staff work at the forefront of basic, translational, and preclinical science, with a focus on cancer, AIDS, and other infectious diseases.
We collaborate with colleagues across the National Cancer Institute, National Institutes of Allergy and Infectious Diseases, and others throughout the National Institutes of Health. We also engage with extramural investigators in academia, government and industry.
Your path to joining our team begins with the desire to work for the only national laboratory dedicated to biomedical research. Our employees share a common desire to help make a difference in cancer research and public health concerns. As you search for a career that fits your education, skills, and abilities, explore the core values that guide us and emphasize work-life balance.
Discover why joining the Frederick National Laboratory team could be the most important career step you take
- Website
- https://frederick.cancer.gov/
- Phone
- 301-846-1000
- Location
-
8560 Progress Drive
Frederick
MD
21701
US
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