Amgen

Sr Associate Scientist - Bioassay and Profiling

Employer
Amgen
Location
Cambridge, MA, US
Posted
Aug 09, 2019
Ref
R-79161
Required Education
Bachelors Degree
Position Type
Full time
The Therapeutic Discovery organization is seeking a highly motivated and capable Sr Associate Scientist to join the Bioassay & Profiling group within the Discovery Technologies department in Cambridge, MA (Amgen Massachusetts-AMA). The successful candidate will be a bench scientist that contributes hands-on to our assay development, implementation, and execution efforts in support of Amgen's small molecule drug discovery pipeline in collaboration with multiple therapeutic areas.

Position responsibilities include:

The development and optimization of novel cell-based assays

ensure successful implementation and routine execution of these assays to support HTS, lead optimization, and mechanism-of-action studies for promising therapeutic targets

Design and execute laboratory experiments, generate and interpret data supporting the successful development of new assays and test the biological effects of therapeutic agents

Design, implement, and optimize gene-editing (e.g., CRISPR) workflows in a manner that enables development and evaluation of real-time, live, cell-based functional assays supporting therapeutic target screening or lead optimization studies

Learn, apply, and incorporate automation solutions during assay development, assay troubleshooting, mechanism-of-action and modes of inhibition analysis, data analysis, and reporting

Investigate, identify, develop, and optimize new methods/techniques to generate reliable and reproducible data promptly

Semi-independently plan and execute experiments, adapt readily to changing project needs and develop sophisticated problem-identification and problem-solving skills

Maintain fluency with the latest scientific literature and rationale surrounding drug targets and be an active contributor to the interpretation of results in group discussions

Show excellent teamwork and communication skills as this role requires an ability to work closely with other scientists in the group and across Amgen

Adherence to good laboratory conduct, documentation practices, and safety guidelines

Basic Qualifications

Doctorate degree OR Doctorate degree completed by August 2019

OR

Master's degree and 4 years of Scientific experience

OR

Bachelor's degree and 6 years of Scientific experience

Preferred Qualifications

Ph.D. degree with 1+ years of post-doctoral training or a Master's degree with 4+ years postgraduate experience in cell and/or molecular biology or closely related discipline

Experience working as part of a multi-disciplinary team in a biopharmaceutical setting

Familiarity with assay technologies including luminescence, fluorescence and FRET/BRET with experience in the utilization of lab automation across assay development and screening

Ability to design and implement gene editing (CRISPR knock out/in) workflows enabling the development of target-specific and disease-relevant cell-based assays

Track record of developing, implementing, and executing novel cell-based screening assays in (384 and/or 1536 well plates) to support early drug discovery research

Ability to create and establish priorities, manage work plans, and meet timelines

Capacity to identify, evaluate, implement, and become proficient in novel laboratory techniques

A flexible mindset with strong interpersonal, communication and problem-solving skills

Demonstrated initiative, teamwork, collaboration and passion for developing technical expertise across assay development to routine execution

Strong scientific foundation in the understanding and implementation of a wide range of cell-based assays and readouts (e.g., TR-FRET, BRET, PCA, AlphaScreen, high content imaging, luminescence and fluorescence-based reporter and/or label-free assays)

Broad experience and expertise in molecular reagent generation and cell line engineering using gene editing approaches (CRISPR) as well as development and implementation of novel cellular target engagement or activity assays (e.g., second messenger generation, ion flux, reporter, NanoBit/BRET, gene activation, cellular imaging)

Experience with cell characterization and analytical techniques such as DNA sequencing, qPCR and protein expression as well as expertise with phenotypic and functional characterization of immortalized or primary cells

Established capabilities in setting-up and operation of fluorescence/luminescence plate readers (e.g., FLIPR, Envision) and automated liquid handlers (e.g., Bravo, Echo, Tempest) in the development of 384- and/or 1536-well microtiter plate assays

Familiarity with software and statistical platforms for the analysis of large data sets (e.g., Screener, Spotfire, MODDE pro/DoE, Pipeline Pilot)

Amgen focuses on areas of high unmet medical need and leverages its expertise to strive for solutions that improve health outcomes and dramatically improve people's lives. A biotechnology pioneer since 1980, Amgen has grown to be one of the world's leading independent biotechnology companies, has reached millions of patients around the world and is developing a pipeline of medicines with breakaway potential.

Amgen is committed to unlocking the potential of biology for patients suffering from serious illnesses by discovering, developing, manufacturing and delivering innovative human therapeutics. This approach begins by using tools like advanced human genetics to unravel the complexities of disease and understand the fundamentals of human biology.

Join Us

If you're seeking a career where you can truly make a difference in the lives of others, a career where you can work at the absolute forefront of biotechnology with the top minds in the field, you'll find it at Amgen.

Amgen, a biotechnology pioneer, discovers, develops and delivers innovative human therapeutics. Our medicines have helped millions of patients in the fight against cancer, kidney disease, rheumatoid arthritis and other serious illnesses.

As an organization dedicated to improving the quality of life for people around the world, Amgen fosters an inclusive environment of diverse, ethical, committed and highly accomplished people who respect each other but compete intensely to win. Together, we live the Amgen values as we continue advancing science to serve patients.

Amgen is an Equal Opportunity employer and will consider all qualified applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, or disability status.