Scientist, microbial fermentation protein sciences and early stage CMC process development

Location
92037, La Jolla
Posted
Jul 19, 2019
Ref
Synthorx
Required Education
Doctorate/PHD/MD
Position Type
Full time

Synthorx is a pharmaceutical company using a novel synthetic biology platform for the discovery and development of transformational protein therapeutics.  We are hiring a driven Scientist with strong background in microbial protein expression and a working knowledge of protein biochemistry. This candidate will be responsible for the development of protein expression processes using Synthorx E.colirecombinant platform to support lead candidate selection, pre-clinical studies and technical transfer to external GMP manufacturing partners. We are seeking a self-starting, highly independent and collaborative individual, able to operate at high pace to meet aggressive timelines.

Key Responsibilities:

  • Perform all steps of protein expression and scale-up. Experience with microbial fermentation is required. Experience with E.coli fermentation is highly desirable.
  • Experimentally determine methods to optimize yields and other process parameters for protein expression.
  • Experience with recombinant production of polypeptides.
  • Lead fermentation process optimization using a data driven approach.
  • Experience with fermentation equipment at bench, pilot and industrial scale. 
  • Collaborate with platform and downstream process development teams to ensure efficient integration of fermentation and upstream and downstream functions.
  • Develop standard operating procedures, document protocols and technology transfer. 
  • Support internal lead discovery efforts and pre-clinical studies.
  • Supervise/Mentor junior staff and provide insight and technical expertise for projectprogression
  • Maintain a high standard for quality control including up-to-date data records for all protein deliveries.
  • Troubleshoot challenging technical, experimental, and instrumental issues.

Required:

  • PhD degree with a major in biochemistry, biochemical engineer, microbial physiology, biology or related field and 3+ or MSc and 10+ years of hands-on experience with recombinant protein production.
  • Experience with E. coli-based protein expression including scale-up preparation employing fermentation instrumentation and protocols.
  • Strong understanding of microbial physiology and its impact on process optimization.
  • Experience with platform systems for incorporation of novel amino acids into polypeptides is a plus.
  • Experience with DOE methodology for improving fermentation titers is highly desired
  • Experience in protein characterization (SDS PAGE, Western Blotting, HPLC). Understanding of MS methods for QC is a plus.
  • Familiarity with protocols involving extraction from inclusion bodies and refolding is a plus.
  • Hands-on familiarity with standard molecular biology methods.
  • Regular activities require ability to communicate effectively and rapidly reprioritize to match dynamic and demanding timelines.

Travel: 0-20%

Additional preferred skills:

  • Experience in technical transfer to external CMOs.
  • Experience in regulatory submissions.