Senior Associate Scientist, Affinity and Kinetics

Employer
Celgene
Location
Seattle, Washington, United States
Posted
Jul 16, 2019
Ref
1901527
Required Education
Bachelors Degree
Position Type
Full time
Req #: 1901527
Location: Seattle, Washington, United States
Job Category: Research and Early Development
Work Location: 400 Dexter Ave N. 98109
Organization: Translational Medicine
Employee Status: Full-time
Job Type: Regular

Summary:

Celgene is looking for a candidate to join the Protein Sciences team within the Immuno-Oncology and Cellular Therapy Department in Seattle.

What great looks like in this role:

Our ideal candidate will be an experienced and independent self-starter and is able to think outside of the box. You have a deep understanding of and experience with SPR (Biacore), basic biochemistry principles, protein structure, and extensive laboratory experience with development and implementation of assays analyzing protein-protein, as well as protein-cell interactions. In this role, you will be responsible for the deployment of kinetics instruments (Biacore and KinExA) to better understand Cellular Therapies developed at Celgene. In addition, you will independently evaluate new biophysical methods and technologies to deepen the understanding of CAR-T cell biology. This role provides a unique opportunity to combine basic protein biochemistry with advanced biophysical instrumentation to understand a new and exciting field of cancer therapy.

Objective of this role
  • Development of custom affinity assays and biochemical assays (ELISA, immune precipitation)
  • Daily maintenance and operation of SPR (Biacore) and Kinetic Exclusion Assay (KinExA) instrumentation
  • Delivery of high quality kinetics data and epitope binning data using a wide range of protein/cellular reagents

Key Responsibilities:

  • Design, and implementation of kinetic assays using cells and recombinant proteins
  • Routine buffer and sample preparation of reagents used in kinetics assays
  • Record experimental details and maintain laboratory notebook in accurate and timely manner
  • Cultivate positive and productive working relationships with colleagues
  • Prepare and present work at group meetings
  • Participate in maintenance of routine laboratory instrumentation and supplies

Minimum Qualifications:

  • Bachelor's degree in Biochemistry (or relevant discipline) and minimum of 8+ years of pharmaceutical laboratory experience required; or Master's degree and minimum of 6+ years of pharmaceutical laboratory experience required;
  • In depth knowledge of and demonstrated expertise operating SPR (Biacore) instrumentation
  • Deep understanding of principles of chemistry, biochemistry, as well SPR and kinetic exclusion assay experimental design
  • Thorough understanding of affinity, avidity, and kinetics concepts
  • Demonstrated ability to develop novel kinetics assays using traditional and non-traditional protein reagents (mAb, scFv, Fc-Fusion, bi-specific antibodies, recombinant receptors, crude serum, VLPs).
  • Experience with epitope binning of antibody and antibody like molecules
  • Ability to multitask, independently organize time and plan specifics of work
  • Excellent communication and interpersonal skills demonstrated in a team environment
  • A strong team player that brings people along through a supportive, engaged and creative way of working
  • Excellent communication and presentation skills.

Preferred Qualifications:

  • Experience handling cells is highly preferred
  • Ability to perform protein purification and modification is highly preferred
  • Hands on experience operating kinetic exclusion assay (KinExA) instrumentation
  • Ability to work independently but also in a collaborative manner with local and cross-site teams
  • Strong understanding of protein folding and structure


About Us

COMMITTED TO IMPROVING THE LIVES OF PATIENTS WORLDWIDE

At Celgene, we seek to deliver truly innovative and life-changing drugs for our patients. Our vision as a company is to build a major global biopharmaceutical corporation while focusing on the discovery, the development, and the commercialization of products for the treatment of cancer and other severe, immune, inflammatory conditions.

"At Celgene, we seek to deliver truly innovative and life-changing drugs for our patients."

There are more than 300 clinical trials at major medical centers using compounds from Celgene. Investigational compounds are being studied for patients with incurable hematological and solid tumor cancers, including multiple myeloma, myelodysplastic syndromes, chronic lymphocytic leukemia (CLL), non-Hodgkin's lymphoma (NHL), triple-negative breast cancer and pancreatic cancer. As committed as we are to clinical accomplishment, we are equally committed to patient support, which is a guiding principle at Celgene. We believe all who can benefit from our discoveries should have the opportunity to do so. Celgene puts patients first with industry-leading programs that provide information, support and access to our innovative therapies.