Scientist - fibrillizing protein reagent characterization

Location
Tarrytown, NY, United States
Posted
Apr 18, 2019
Ref
15546BR
Required Education
Doctorate/PHD/MD
Position Type
Full time
Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

Summary
The position will primarily focus on the production & characterization of aggregated, 'fibrillizing protein' reagents which are related to neurodegenerative 'proteinopathies'. Examples would be Tau, α-synculcein, TTR, & TDP-43 proteins. The person will develop methods to isolate the different forms of protein aggregates (monomer, oligomer, fibrils, etc.), investigate parameters which affect the misfolding of fibrillizing protein, & generate inventories of these proteins for research programs.

Responsibilities

• Protein expression in E.coli, optimization of expression, & scale-up

• Purify recombinant protein using ÄKTA chromatography equipment with methods such as affinity, ion exchange, size exclusion, & reverse phase

• Organize & present data for weekly meetings & for larger, departmental groups

• Carry out large scale production of fibrillizing proteins

• Process fibrillizing protein mixtures to isolate & characterize the different sizes & forms of aggregates & then create inventories of the isolated aggregate forms

• Collaborate with other groups to share data/results & tailor experiments to meet project goals

• Keep up-to-date with current literature in the field to further develop scientific expertise & stay aware of important findings which
can be used in our research
#LI-CB1

Requirements
1-2 years research experience focused on neurodegenerative 'proteinopathies'
Protein expression in E.coli
Protein purification by affinity, ion exchange, reverse phase, & SEC chromatography
Experience running ÄKTA chromatography equipment
SDS-Page electrophoresis, Western blotting, & spectrophotometry
Sterile techniques
Good Lab practices
Documentation of experiments & results in an electronic LIMS notebook
Ph.D. required

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.