R&D Specialist - Reagent Production

Location
Tarrytown, NY, United States
Posted
Apr 01, 2019
Ref
15545BR
Required Education
Bachelors Degree
Position Type
Full time
Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

Summary
The position will primarily focus on the production of reagents associated with T-cell receptors. The individual will express recombinant HLA & TCR proteins in E. coli, & then refold & purify the resulting complexes. Additionally, the person will also express, purify, & characterize other recombinant proteins which support multiple targets within Regeneron's pipeline.

Responsibilities

• Express recombinant protein in E.coli, optimization of expression, & scale-up

• Cell lysis & clarification, isolation of inclusion bodies, solubilization of HLA-related proteins

• Solubilization & handling of peptides

• Refold & purify HLA/TCR complexes; biotinylation/conjugation of complexes, & labeling with fluorescent tags

• General protein purification with ÄKTA chromatography equipment & using affinity, ion exchange, size exclusion, & reverse phase methods

• Carry out experiments to improve protein solubility & stability

• Work collaboratively with other researchers to plan, update, & stay abreast of deadlines

• Documentation of experiments & results in an electronic LIMS notebook
Requirements

• 1-2 years applied experience with protein expression in E.coli & protein purification using affinity, ion exchange, reverse phase, & size exclusion techniques

• Experience running ÄKTA chromatography equipment

• SDS-Page electrophoresis, Western blotting, & spectrophotometry

• Sterile techniques

• Good Lab practices

• Data entry and notebook keeping using ELN

• Bachelors Degree required

This position will require the individual to pass a physical before beginning employment.

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.