R&D Specialist - Immune Cell Functional Bioassay Development 1 of 2

Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

Summary
The Bioassay Group in the Therapeutic Proteins Department seeks a highly motivated and passionate Research Associate to join a fast-paced, dynamic group involved in cell-based assay development and high-throughput screening in early oncology and immunology drug development. The ideal candidate should have experience developing, optimizing, and troubleshooting bioassays for small molecules, biologics, and/or antibody drug conjugates. Additional experience running immune cell functional assays such as T-cell proliferation, T cell cytotoxicity, cytokine release assays is required. Strong multitasking and communication skills, attention to detail, and the ability to collaborate across groups in a fast-paced team environment is essential.

Responsibilities
1) Develop, optimize, and execute cell-based assays in 96 and 384 well formats (viability/cytotoxicity, luciferase reporter, membrane potential, and internalization assays)
2) Screen small molecules, biologics, and antibody drug conjugates for functional activity using cell-based assays in 96 and 384 well formats
3) Cell culture of adherent/non-adherent immortalized cell lines and human primary cells
4) Cell line engineering through lentiviral or other transfection techniques
5) Flow cytometry acquisition and analysis both for immune cell identification and expression profiling, and for the generation of antibody binding curves
6) Operate a variety of instruments including but not limited to iQue, Accuri C6 cytometer, Cytoflex, Opera Phenix, ImageXpress micro XL, Victor, Envison, Ensight, FLIPR, Perkin Elmer Health Sciences MicroBeta M2 scintillation plate reader, and Hamilton STAR
7) Provide Weekly presentations/verbal updates
Requirements
1. Bachelors (5+ years) or Masters (2+ years)
2. Experience working with human blood cells
3. Strong aseptic cell culture skills
4. Flow cytometry expertise
5. Immunofluorescence staining
6. Cell-based bioassay development and screening in 96-well or 384-well plates
7. Isolation/separation of distinct immune cell population from whole blood
8. Excellent communicator
9. Ability to multi-task on several different projects
10. Exceptional organization, multi-tasking, and record keeping skills

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.