Research Scientist In-Vivo Pharmacology
The qualified candidate is a highly motivated, interactive, and creative individual that possesses the ability to work across a highly matrixed environment to advance preclinical drug development programs from target identification/validation through IND enabling activities. The candidate will be expected to effectively collaborate with colleagues in the Discovery Oncology, immunology, protein engineering/antibody development and medicinal chemistry groups. The successful candidate will demonstrate clear and professional verbal and written communication, capable of presenting scientific results to multidisciplinary teams and key stakeholders.
- A B.S. with a minimum of 7 years, or an M.S. with a minimum of 5 years of industrial experience with strong scientific knowledge and research experience in in vivo pharmacology, cancer biology, immuno-oncology, or immunology is highly desirable.
- Extensive knowledge of preclinical drug development including knowledge of independently designing and conducting in vivo and ex vivo experiments to identify and profile drug candidates is required.
- Demonstrated independence in designing, executing, and analyzing data from in vivo studies with tumor bearing mice is required.
- Experience with syngeneic tumor models is preferred; experience with GEMMs is a plus.
- Required skills include: model development, tumor implantation, animal identification and randomization, dosing via IV, IP, SC, I-TUMOR and PO routes, tumor measurements by caliper, blood draws by tail vessel micro-sampling, cardiac puncture and tissue/tumor collection at necropsy including fixation, freezing and tissue disaggregation to support ex vivo analyses
- Proficiency in designing multi-color FACS panels and using them to characterize tumor infiltrating lymphocytes is very highly desirable.
- Proficiency in cell culture and ex vivo techniques such as qPCR, ELISA (MSD), or WB is required.
- Experience with surgical procedures to enable development of orthotopic tumor models and using ultrasound to monitor orthotopic tumor growth is desirable.
- Experience conducting in vivo pooled genetic screens is desirable.
- Proficiency in utilizing software such as Graph Pad, FlowJo, Microsoft office etc. for data analysis and figure generation is required.
- Ability to mentor junior scientists and colleagues on best practices in the laboratory would be highly valued.
- Attention to detail with excellent organizational and record keeping skills is a requirement.
- Ability to thrive as a member of a diverse and motivated team of researchers spanning across the Oncology Discovery group is a requirement.
- Independently design and conduct mouse in vivo pharmacology experiments, including but not limited to model development, tumor implantation, animal identification and randomization, dosing via IV, IP, SC, I-TUMOR and PO routes, tumor measurements by caliper, weighing, health monitoring, tissue sampling by fine needle biopsy and core needle biopsy, peritoneal lavage, blood draws by retro-orbital, tail vessel micro-sampling, cardiac puncture and tissue/tumor collection at necropsy including fixation, freezing and tissue disaggregation to support ex vivo analyses
- Mentor junior associates on best practices (in vivo and ex vivo)
- Establish complex orthotopic tumor models
- Utilize ELISAs (MSD), qPCR, or WB to monitor biomarkers in harvested tissues
- Lead or contribute to the utilization of multi-color flow cytometry to profile the immune infiltrate in harvested mouse tissues.
- Accurately maintain proper scientific documentation associated with pharmacology studies in an electronic lab notebook.
- Effectively communicate and present summaries of research results to team members or to a multidisciplinary team of researchers.
- Work as a member of a diverse and motivated team of researchers spanning across the Oncology Discovery group and other line functions.
- Rotating weekend, holiday and off-hour on-site responsibilities to support BID/QD dosing will be expected of this position.
- This position is located in Cambridge, MA. There will be less than 10% travel.