Associate Scientist/Scientist, Upstream Process Development

Location
92037, La Jolla
Posted
Feb 14, 2019
Required Education
Doctorate/PHD/MD
Position Type
Full time

Synthorx is a pharmaceuticals startup company using a novel synthetic biology platform for the discovery and development of transformational protein therapeutics. We are hiring a driven Associate Scientist/Scientist with strong background in microbial protein expression and a working knowledge of protein biochemistry. This candidate will be responsible for the development of protein expression processes using Synthorx E.colirecombinant platform to support lead candidate selection, pre-clinical studies and technical transfer to external GMP manufacturing partners. We are seeking a self-starting, highly independent and collaborative individual, able to operate at high pace, to meet aggressive timelines.

Key Responsibilities:

  • Perform all steps of protein expression and scale-up. Experience with microbial fermentation is required.
  • Experimentally determine methods to optimize yields and other process parameters for protein expression
  • Experience with production of polypeptides employing recombinant systems
  • Leads fermentation process optimization using a data driven approach.
  • Collaborate with platform and downstream process development teams to ensure efficient integration of fermentation and upstream and downstream functions
  • Develop standard operating procedures, document protocols and technology transfer
  • Support internal lead discovery efforts and pre-clinical studies
  • Prepare and present project updates to ensure timely delivery
  • Maintain a high standard for quality control including up-to-date data records for all protein deliveries, including HPLC and basic understanding of mass spectrometry methods for QC
  • Troubleshoot challenging technical, experimental, and instrumental issues

Required:

  • PhD degree, with a major in biochemistry, biochemical engineer, microbial physiology, biology or related field or MSc and 5+ years of hands-on experience with recombinant protein production
  • Experience with E. coli-based protein expression including scale-up preparation employing fermentation instrumentation and protocols.
  • Strong understanding of microbial physiology and its impact on process optimization
  • Experience with DOE methodology for improving fermentation titers is highly desired
  • Experience in protein characterization (SDS PAGE, Western Blotting, HPLC). Understanding of MS methods for QC is a plus
  • Familiarity with protocols involving extraction from inclusion bodies and refolding is a plus
  • Hands-on familiarity with standard molecular biology methods
  • Regular activities require ability to communicate effectively and rapidly reprioritize to match dynamic and demanding timelines

Additional preferred skills:

  • Basic understanding of cancer biology and/or immunology