Research Associate, Protein Production Process Development

Location
92037, La Jolla
Posted
Feb 14, 2019
Required Education
Bachelors Degree
Position Type
Full time

Research Associate/Senior Research Associate with background in E. coliprotein expression and a working knowledge of protein biochemistry and purification. 

Key Responsibilities:

  • Carry out experiments and collect, process data under supervision of the scientist.
  • Perform all steps of protein expression and purification.
  • Familiarity with protocols involving extraction from inclusion bodies and refolding is a plus.
  • Collaborate cross-functionally with Protein Sciences teams to ensure smooth continuity between upstream and downstream technology processes.
  • Develop standard operating procedures and document protocols.
  • Prepare and present project updates to ensure timely delivery.
  • Maintain a high standard for quality control including up-to-date data records for all protein deliveries.
  • Troubleshoot challenging technical, experimental, and instrumental issues.
  • Consummate ability to work in a highly collaborative, fast-paced, cross-functional environment, with superior oral and written communication skills.

Required:

  • BS or MSc degree, with a major in biochemistry, biology or related field, and 2+ years of hands-on experience with recombinant protein production.
  • Experience with E. coli-based protein expression. 
  • Experience developing methods for protein purification. Familiarity with protocols involving extraction from inclusion bodies and refolding is a plus.
  • Chromatography by affinity, IEX, and SEC. Familiarity with AKTA instrumentation is highly desirable.
  • Experience in protein characterization (SDS PAGE, Western Blotting, quantification). 
  • Hands-on familiarity with standard molecular biology methods.
  • Regular activities require ability to communicate effectively and rapidly reprioritize to match dynamic and demanding timelines.