Research Associate I, RAS/RAF Biology

The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives. The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community. A major focus of the CRTP is the NCI RAS Initiative with the goal to discover new therapeutic interventions against RAS-driven cancers. In addition, the CRTP hosts the Nanotechnology Characterization Laboratory (NCL) which performs and standardizes preclinical efficacy and toxicity testing of nanoparticles intended for cancer therapeutics and diagnostics, and the Antibody Characterization Lab (ACL) which characterizes monoclonal antibodies or other renewable affinity binding reagents for use in cancer related research. CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.

KEY ROLES/RESPONSIBILITIES

The RAS Imaging and Assay Development Group provides support for the RAS Initiative in a highly interactive, team-based environment to develop therapeutic strategies for targeting the oncoprotein RAS, and for developing a deeper understanding of RAS structure and function in biological membranes. The Research Associate I (RAI) will:

• Carry out experiments in cell biology, under review of a scientist, including the use of advanced fluorescence microcopy
• Cell culture, cell line maintenance, live cell handling and related techniques
• Perform plasmid transfections in mammalian cell lines
• Perform western blots to evaluate cell signaling events in mammalian cells
• Provide timely updates of project progress to supervisor and other staff in both informal and formal reports
• Maintain detailed records of all laboratory processes and procedures for quality assurance purposes

BASIC QUALIFICATIONS

• Possession of a B.S. degree from an accredited college or university according to the Council for Higher Education Accreditation. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency.
• In addition to the educational requirements, a minimum of two (2) years of related experience is required
• Must be able to obtain and maintain a clearance

PREFERRED QUALIFICATIONS

• Confocal and/or TIRF microscopy on live or fixed cells
• Single molecule microscopy and various fluorescence imaging techniques
• PCR and other molecular biology techniques
• Prior hands-on experience with quantitative western blot and Co-IP technique is highly desired
• Proficiency with quantitative image analysis software and programming in MatLab is advantageous

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)