Circulating Tumor Cell Methods Developer
- Employer
- Frederick National Laboratory for Cancer Research
- Location
- Frederick, MD, USA
- Start date
- Dec 25, 2018
View more
- Discipline
- Science/R&D, Oncology
- Required Education
- Bachelors Degree
- Position Type
- Full time
- Hotbed
- BioCapital
Job Details
KEY ROLES/RESPONSIBILITIES
The Research Associate III will:
- Develop and validate new methods for the isolation, enumeration and biomarker analysis of circulating tumor cells (CTCs)
- Develop biomarker assays to evaluate circulating tumor cells in liquid specimens from cancer patients and from mice harboring patient-derived tumors using modern technology platforms
- Develop and implement analyses of CTCs using multiplex immunofluorescence assays using confocal microscopy, whole slide imaging or imaging cytometry in support of clinical studies and initiatives
- Perform both routine and experimental analysis of human and animal blood samples and tumor cell lines at the level of individual cells
- Perform data analysis of results from immunofluorescence assay studies on preclinical and clinical samples
- Design, test, and write detailed technical SOPs for imaging and CTC analysis for assay transfer to external labs
- Generate materials and data necessary to support the primary experimental objectives of the program
- Maintain instrumentation, computer databases, and assure regulatory and safety compliance within the laboratory
- Perform extensive benchwork with advanced automated instrumentation
BASIC QUALIFICATIONS
To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:
- Possession of a Bachelor's degree from an accredited college or university according to the Council for Higher Education Accreditation (CHEA) or four (4) years equivalent experience in the appropriate field in lieu of degree.
- Foreign degrees must be evaluated for U.S. equivalency
- Candidate must have completed laboratory science courses in two or more of the following subjects: histology; chemistry; biochemistry; immunology; pharmacology; cell biology; hematology
- In addition to educational requirements, a minimum of eight (8) years of related biomedical experience
- Must be able to obtain and maintain a security clearance
PREFERRED QUALIFICATIONS
Candidates with these desired skills will be given preferential consideration:
- Four (4) or more years relevant experience in processing and analysis of blood specimens from pre-clinical and clinical models
- Experience with the isolation and analysis of CTCs from clinical or preclinical specimens
- Experience in cell-based analysis and assay development
- Day-to-day experience in managing laboratory infrastructure in a GxP environment
- Quantitative laboratory work experience one of the following areas: biochemistry, cell biology, chemistry, biotechnology, and/or histopathology
EXPECTED COMPETENCIES
- Ability to develop, modify methods and techniques independently, under supervision of a lead scientist
- Ability to maintain precise records. Must be able to provide accurate scientific reports including details of experimental procedures, results and conclusions to supervisor
- Demonstrated expertise in aseptic cell culture and maintenance of multiple different cell lines without contamination
- Demonstrated expertise in drug and chemical preparation
- Hands on experience in two or more basic cell biology techniques is essential: antibody-based assays, immunofluorescence assays, cell lysate extraction, western-blotting, 96-well plate ELISAs.
- Hands on experience with one or more of the following cell analysis techniques: immunofluorescence; confocal microscopy; imaging flow cytometry such as Amnis; CTC analyzers such as Cell Search or ApoStream; Incell analyzers; fluorescence microscopy; protein concentration determination assays, single cell analysis, cellular imaging
- Experience performing In vitro cell line testing using multiple test agents and drug combinations of interest to determine drug dose responses, biomarker feasibility, timing of marker activation.
- Ability to recognize when scientific findings deviated from expected results and how to distinguish discoveries from suspect results
- Ability to perform experiments in compliance with/under control of an SOP
- Ability to manage work on multiple projects simultaneously, and to adapt to frequent changes in project priorities
- Ability to quickly master new and novel measurement technologies and instrumentation
- Excellent written and spoken English language and computer skills (essential)
- Proficiency with Microsoft Office software (Outlook, Excel, Word, and PowerPoint)
Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)
Company
A rewarding career with global impact
Whether you’re an expert in your field or just starting out, we have a career opportunity for you. We’re always looking for people to join us in fulfilling the mission of the Frederick National Laboratory: discovery, innovation, and success in the biomedical sciences.
Our team of 2,400+ scientists, technicians, administrators, and support staff work at the forefront of basic, translational, and preclinical science, with a focus on cancer, AIDS, and other infectious diseases.
We collaborate with colleagues across the National Cancer Institute, National Institutes of Allergy and Infectious Diseases, and others throughout the National Institutes of Health. We also engage with extramural investigators in academia, government and industry.
Your path to joining our team begins with the desire to work for the only national laboratory dedicated to biomedical research. Our employees share a common desire to help make a difference in cancer research and public health concerns. As you search for a career that fits your education, skills, and abilities, explore the core values that guide us and emphasize work-life balance.
Discover why joining the Frederick National Laboratory team could be the most important career step you take
- Website
- https://frederick.cancer.gov/
- Phone
- 301-846-1000
- Location
-
8560 Progress Drive
Frederick
MD
21701
US
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