Scientist I

Frederick, MD, USA
Nov 29, 2018
Required Education
Position Type
Full time
The Basic Science Program (BSP) pursues independent, multidisciplinary research in basic and applied molecular biology, immunology, retrovirology, cancer biology, and human genetics. Research efforts and support are an integral part of the Center for Cancer Research (CCR) at the Frederick National Laboratory for Cancer Research (FNLCR). The Mouse Cancer Genetics Program, Hematopoiesis & Stem Cell Biology Section, under the direction of Dr. Jonathan Keller, studies the transcriptional regulation of stem cell quiescence, survival, self-renewal, and differentiation. The goal is to understand how commitment to specific lineages is programmed and cell specific patterns of gene expression are established. Current studies are focused on defining the role of transcriptional regulators in myeloid cell development and their potential role in leukemogenesis.

  • The Scientist I will support research efforts to define the role of transcriptional regulators in myeloid cell development, and their potential role in leukemogenesis.
  • This work will be accomplished performing both molecular and stem cell biology techniques, cloning and construction of lentiviral and retroviral vectors, quantitative RT-PCR, analysis of transcription factor function (EMSA and CHIP assays), and experience working with hematopoietic cells in vitro and in vivo.
  • The Scientist I will breed, type and maintain transgenic, conventional and targeted KO mouse strains.
  • The Scientist I will sort bone marrow stem cells by flow cytometry and oversee all bone marrow transplantation studies including tracking donor cells by flow cytometry and evaluating gene expression by WB analysis and PCR.
  • The scientist will be responsible for mentoring and training post-doctoral fellows and students in the laboratory.
  • The Scientist I will assist the PI in preparing figures and writing and interpreting the results of experiments.

  • Possession of a doctoral degree from an accredited college or university according to the Council for Higher Education Accreditation. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency)
  • No work experience is required beyond the doctorate degree
  • Must be able to obtain and maintain a security clearance

  • Experience or knowledge in the following:
  • Hematopoiesis and hematopoietic stem cells (HSCs)
  • Flow cytometry cell sorting and analysis of HSCs and their progeny
  • Maintaining transgenic and mutant mouse strains
  • Molecular biology techniques including cloning, RNA-seq, single cell analysis, Q-RT-PCR
  • Lentiviral and retroviral vector construction and transduction
  • Bone marrow transplantation

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)