Associate Scientist, T Cell Gene Editing

Location
Cambridge, MA, United States
Posted
Nov 22, 2018
Ref
1214747
Hotbed
Genetown
Required Education
Bachelors Degree
Position Type
Full time
Join bluebird bio's Cambridge-based genome editing team to help bring innovative immunotherapy products into the clinic. In this role, you will contribute to the pre-clinical and clinical development of gene-edited CAR/TCR T cells for oncology indications. An ideal candidate should have a background in Immunology, ideally in T cell biology, and a strong interest in genome editing. The candidate should also possess excellent communication and organizational skills, be able to work in a fast-paced, highly collaborative environment, and be passionate about developing innovative cancer therapeutics that can transform patient's lives.

About the role:
  • Develop and optimize protocols for T cell gene editing, including: megaTAL delivery methods, culture conditions, scale up, and multiplexing
  • Characterize genetically modified primary T lymphocytes using standard immunological assays including flow cytometry
  • Develop and perform immune cell activation, cytotoxicity, and other cellular and ELISA-based assays to evaluate gene editing approaches that improve the efficacy of CAR and TCR based T cell therapeutics in vitro and in vivo
  • Maintain an electronic lab notebook and contribute to support documentation when necessary
  • Contribute to experimental design and project direction
  • Provide critical lab support while working in a collaborative team environment
  • Compile and present research data to functional teams


About you:
  • BS in immunology, cell biology or related discipline with 2-4 years of experience or MS with 0-2 years experience (industry experience preferred)
  • Good understanding of immunology and cancer biology
  • Experience in primary human immune cell isolation techniques and mammalian cell culture, including cell culture of primary human T lymphocytes
  • Experience working in in vitro immunology studies and functional T cell assay development using techniques such as multi-color flow cytometry, Luminex, ELISA and cytotoxicity assays is required
  • Experience with gene editing or lentiviral vector transduction a plus
  • Experience with standard molecular biology techniques, including but not limited to, PCR, RT-PCR, qPCR, Westerns, and RNA in vitro transcription a plus
  • Excellent computer skills, including experience with Microsoft Excel and PowerPoint, Graphpad Prism, DNA software tools (e.g. Geneious), and FlowJo
  • Ability to quickly adapt to new research areas and implement new techniques independently
  • Scientifically rigorous, highly organized, and with significant attention to detail
  • Strong oral and written communication skills
  • Self-motivated and enthusiastic about working in an interdisciplinary, collaborative research environment
  • Be ready to embrace the principles of the bluebird bio culture: b colorful, b cooperative, b yourself