Senior Associate Scientist, Protein Engineering

Location
Seattle, WA, United States
Posted
Nov 22, 2018
Ref
1150204
Required Education
Bachelors Degree
Position Type
Full time
Seattle-based Oncology Discovery Research team

Join bluebird bio's cutting-edge oncology discovery research team developing novel technologies to enhance the success of our gene therapy and gene editing programs. The successful candidate will join our Protein Engineering team and provide critical support for ongoing and emerging research projects. Activities will include protein construct design and cloning, DNA library preparation and screening, cell based immunological assays, and high-content genomics assays. Opportunities to grow into more advanced molecular engineering projects, sample generation techniques, and analytical assays are abundant.

About the position:
  • Creative protein design and optimization
  • Develop cell-based assay for throughput screening
  • Immunological assays including CBA, ELISA and flow cytometry
  • Lentiviral vector generation to genetically modify primary human cells
  • Molecular cloning and plasmid production
  • Gel electrophoresis, western blotting and fragment analysis
  • Protein expression and purification
  • Primary cell culture and aseptic technique
  • Next generation sequencing library prep and analysis
  • Database entry and maintenance
  • Compile and present research data to functional teams
  • Provide lab support while working in a collaborative team environment

About You:
  • BS/MS degree in biology, cell biology, microbiology, immunology, biochemistry, bioengineering, biophysics or related discipline
  • 3+ year track record of lab experience with a protein biochemistry and immunology focus
  • Competency in basic molecular biology techniques, including primer design, PCR, agarose gel electrophoresis, bacterial transformation
  • Proficiency in cell biology techniques including transfections (mRNA), viral transductions, stable cell line generation and immunological assays
  • Experience in protein purification techniques, surface display technologies and biochemical analyses will be a plus
  • Experience with DNA software tools including with plasmid and sequence analysis (e.g. Geneious) and data analysis programs such as GraphPad Prism
  • Outstanding organizational and record keeping skills
  • Ability to meticulously maintain a lab notebook and contribute to support documentation
  • Be able to work in a highly collaborative team environment
  • Willingness to embrace and demonstrate the core principles of the bluebird bio culture: b colorful, b cooperative, and b yourself