Bristol-Myers Squibb Company

Scientist, HTP Expression Purification (41)

Location
Redwood City, CA, US
Posted
Nov 20, 2018
Ref
R1507900
Required Education
Doctorate/PHD/MD
Position Type
Full time
Scientist - HTP Expression Purification

Job Description:

We are seeking a skilled molecular biologist and protein chemist at a Scientist level to join the high-throughput expression and purification group. The candidate will be expected to work on a variety of projects to purify antibodies from multiple species and heterologous expression systems using automated platforms.

Specific Responsibilities:
• General molecular biology involving isolation, manipulation, and characterization of DNA, RNA and proteins.
• Design of mammalian cell expression vectors for recombinant proteins.
• Mammalian cell culture and transfection using viral and lipid transfection systems.
• Purify antibodies using variety of systems (protein A/G/L and ion exchange).
• Analyze antibodies using automated protein characterization methods (SDS-PAGE/CE-SDS, SEC-MALLS, endotoxin, etc.).
• Clone, express, and purify antibodies using automated systems at small to medium-scale.
• Perform experiments with strong attention to detail and documentation.
• Excellent written and oral skills are necessary.
• Accomplish goals under project team time lines.

Requirements:
• PhD in Biochemistry, Structural Biology, or related field with 0-5 years of industrial experience.
• Demonstrated experience in design and construction of cell expression plasmid constructs, site-directed mutagenesis, and recombinant protein vector design.
• Experience in expression of antibodies from small to medium scale is required.
• Experience using automation and programming jobs on automation workstations is highly desirable.
• Excellent time management, record keeping and organizational skills.
• Demonstrated ability to work in a multidisciplinary team.