Scientist - Cryo Electron Microscopy

Location
Tarrytown, New York, US
Posted
Nov 07, 2018
Ref
13588BR
Required Education
Doctorate/PHD/MD
Position Type
Full time
Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

Summary
We are seeking a PhD-level scientist to join our growing structural biology group. This position will focus exclusively on cryo-electron microscopy of molecules in Regeneron's drug development pipeline, including membrane proteins, large multi-protein assemblies, and antibody-target complexes. This is a tremendous opportunity for an individual to contribute to projects of real medical impact, as well as help establish cryo-electron microscopy as a tool to drive Regeneron's research forward.

Responsibilities
This work will involve many of the stages of a structural biology project, from protein characterization and complex purification, to EM grid preparation, negative stain and cryo-electron microscopy data collection, data processing, structure determination and refinement. Earlier-stage work such as cloning, protein expression, and protein purification will be carried out by other groups at Regeneron, but our group will remain involved in these stages, e.g. when special constructs are required for the structural work.

Although the goal of our efforts will be to produce high-resolution cryoEM structures, it will be important to generate results for the project at each stage of the process, from initial micrographs, to negative stain 2D and 3D reconstructions, to low-resolution cryoEM maps. Many projects will benefit greatly even if a near-atomic resolution structure is not possible.

At the moment, and for the near future, we are traveling to external imaging facilities and using their microscopes to collect our data. However, we will install our own Titan Krios at Regeneron in late 2019, providing nearly unlimited time for data collection on a state of the art microscope. One of the main responsibilities of this position will be to ensure that this microscope is used productively, by generating enough cryoEM samples to "feed the beast", and by processing and analyzing the resulting data. The group is small enough that we will not have a dedicated person to maintain the microscope, so all group members will be responsible for routine maintenance and alignment, with a service contract in place for more significant repairs.

This position will be highly collaborative, interacting with scientists in all areas of Regeneron R&D and beyond. The person hired for this job will need to understand complex research projects involving many different scientific areas, contribute in project meetings to shape the direction of the research, clearly communicate their structural results to an audience of non-structural biologists, and direct the course of their own work without extensive supervision.

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Requirements
This position requires a minimum of a PhD in structural biology, with the thesis research being mostly or completely focused on protein cryo-electron microscopy. Research on an integrated structural biology project (combining cryoEM with other techniques) is acceptable, as long as cryoEM was at the core of the project and represents the majority of the work performed.

2-4 years of postdoctoral experience in protein cryoEM or closely related fields (e.g. cryo-electron tomography) is also highly desirable.

Experience in other structural biology disciplines such as X-ray crystallography or protein NMR is helpful, but cannot substitute for the cryoEM experience.

Since this position will be hands-on in the lab, a substantial amount of experience purifying and characterizing proteins is also necessary.

The successful candidate must have a demonstrated track record of independent research including one or more first-author publications.

This position will involve work on many different projects simultaneously, so excellent record-keeping and time management skills are very important, as well as the ability to absorb new information rapidly. The candidate must be able to work collaboratively with scientists from different backgrounds, and be able to explain their results clearly and concisely to many different audiences, most of whom will have little or no experience in structural biology.

It will be very helpful if the candidate has some direct experience maintaining and aligning electron microscopes, as we have no facility manager and will need to carry out these tasks ourselves (along with a service contract from the manufacturer).

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.