Research Associate II (Histopathology)

Location
Gaithersburg, MD, USA
Posted
Nov 06, 2018
Ref
req152
Discipline
Science/R&D, Research
Hotbed
BioCapital
Required Education
Bachelors Degree
Position Type
Full time
The Cancer Genomics Research Laboratory (CGR) investigates the contribution of germline and somatic genetic variation to cancer susceptibility and outcomes in support of the National Cancer Institute (NCI)'s Division of Cancer Epidemiology and Genetics (DCEG). Working in concert with epidemiologists, biostatisticians and basic research scientists in DCEG's intramural research program, CGR provides the capacity to conduct genome-wide discovery studies and targeted regional approaches to identify the heritable determinants of various forms of cancer. This includes the design and analysis of high throughput studies using various types of “-omics” technologies such as array- and sequence-based genome-wide association studies, studies of tumor characteristics using integrated genomic data analysis, and molecular epidemiologic studies based on novel metabolomic and microbiomic assays.

KEY ROLES/RESPONSIBILITIES

Research Associate II

The Cancer Genomics Research (CGR) Laboratory is a fast-paced, high throughput facility dedicated to the support of genetic and epidemiologic studies for investigators at the National Cancer Institute.

The Research Associate II will:
  • Develop, optimize and troubleshoot immunostaining assays on automated platforms for prospective image analysis
  • Design and execute comparative experiments including preliminary QC/analysis of results, and writing/presenting reports
  • Ensure proper documentation of all experimental and laboratory processes
  • Perform basic histology techniques: embedding, microtomy, H&E staining, coverslipping
  • Digitize H&E and IHC/IF slides using a slide scanner
  • Maintain instruments by use of preventive maintenance procedures
  • Organizes everyday tasks and monitor the inventory for immunostaining projects
  • Train new staff on established SOPs

This position is part of the Molecular Digital Pathology Laboratory (MDPL) at the Cancer Genomics Research (CGR) Laboratory in Gaithersburg, MD

BASIC QUALIFICATIONS
  • Possession of a Bachelor's degree from an accredited college/university in a field related to biomedical research. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S equivalency.
  • A minimum of five (5) years related histopathology experience
  • Extensive experience in chromogenic and fluorescent assay development/optimization on automated staining platforms using human FFPE and frozen tissue sections.
  • Proficiency with automated histo-pathology equipment: tissue processor, microtomes, H&E stainer, slide coverslipper, bright field and fluorescent microscope, stereoscope, IHC stainers.
  • Knowledge of basic histological techniques: processing and embedding, microtomy/cryotomy, H&E staining, slide coveslipping, microscopic recognition of major human tissue types.
  • Must be able to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS
  • Experience with IHC/IF assays on archival human FFPE oncology samples
  • Experience with image analysis platforms
  • Experience with basic molecular biology techniques: RNA, DNA, protein purification, RNAse-free tissue handling of fixed and frozen biological material
  • Experience with ISH/RNAScope and OPAL multiplex assay on automated IHC stainers
  • Working experience with hand macro-dissection and laser capture microdissection of FFPE and frozen samples, TMA construction on automated tissue arrayer, slide scanner
  • Experience with laboratory management system (LIMS)
  • HT/HTL (ASCP) certification by the American Society for Clinical Pathologists

Expected Competencies:
  • Management and proper handling of a diverse collection of FFPE and frozen human biospecimens
  • Strong sense of personal responsibility and ability to work with a team
  • Careful attention to detail. Must be analytical, flexible, innovative, and self-motivated
  • Ability to prioritize, organize and execute multiple projects
  • Ability to troubleshoot immunostaining assays with minimal supervision
  • Ability to train and mentor staff on established SOPs
  • Excellent written and oral communication-ability to communicate clearly and effectively with staff and other groups within CGR and DCEG, and able to interpret data and write scientific reports as well as other written communications
  • Computer literacy, proficiency and aptitude

Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW)