Bristol-Myers Squibb Company

Research Associate, Hybridoma Antibody Generation Scientist (29)

Location
Redwood City, CA, US
Posted
Oct 30, 2018
Ref
R1507921
Required Education
Bachelors Degree
Position Type
Full time
We are seeking an RA with hands-on experience in antibody discovery using hybridoma and/or B-cell approaches to support therapeutic biologics programs and reagent antibody generation. Responsibilities will include executing on all steps of the antibody generation process. This includes preparing immunization, hybridoma fusions, and screening for antibodies.

Responsibilities:

• Contribute to antibody discovery goals utilizing hybridoma and molecular biology approaches
• Participate in designing antibody generation strategies including immunogen design and screening cascades.
• Execute on all stages of antibody generation, including scheduling and preparing immunizations, hybridoma fusion and culture, screening of antibodies, subcloning and hybridoma expansion for sequencing and antibody purification.
• Execute binding screens using ELISA, flow cytometry, or HTRF assays. Experience in high-throughput approaches and/or automation is a plus.
• Execute antibody characterization assays including epitope binning and functional assays.
• Maintain detailed records of all experiments in ELN and using in-house databases and tracking systems.
• This position will require the ability to multi-task and work on several projects simultaneously. Prepare detailed and thorough analysis of data and present updates to managers and project teams.
• Responsible for delivering both data analysis packages as well as purified antibodies to project teams and collaborators.
• Trouble-shoot and optimize protocols and propose creative solutions to improve efficiency to existing processes.
• Communicate with multiple functional areas (e.g. Automation, Proteins groups) as well as project teams to coordinate antibody generation efforts.
• Keep up to date on current protocols and methods for antibody generation and propose new ideas to improve in-house processes

Qualifications:

• BA/BS in Biology, Immunology, or related field with 5+ years' experience.
• Experience with antibody generation using hybridoma approaches and/or B-cell approaches is required
• Understanding of the requirements for therapeutic antibodies properties is a plus. Previous industry experience is a plus.
• Extensive experience with mammalian cell culture and sterile techniques is required.
• Strong experience with antibody binding assays, especially ELISA and multi-color flow cytometry is required
• Experience with BLI/SPR (Octet, Biacore) for epitope binning analysis is a plus.
• Expertise in Excel, Graphpad Prism, and flow cytometry analysis software is required, Spotfire experience is a plus.
• Strong focus, motivation, and commitment to hands-on lab work is required.
• Excellent organization and communication skills.
• Previous experience in PCR, plasmid generation, and transfection of mammalian cells is a plus.
• Previous experience using DNA sequence analysis applications and molecular biology applications (Vector NTI, Geneious) is a plus.
• Ability to multi-task in a fast-paced environment.
• Ability to work and thrive in a collaborative, cross-functional team-oriented