Post Doctoral Fellow

Location
Bethesda, Maryland
Posted
Oct 25, 2018
Ref
req110
Hotbed
BioCapital
Required Education
Doctorate/PHD/MD
Position Type
Full time
LHRI - The Laboratory of Human Retrovirology and Immunoinformatics (LHRI), Clinical Services Program, Applied/ Developmental Directorate, has investigated mechanisms multiple drug resistance (MDR) of HIV and innate immune response against HIV or HIV related virus infection collaborating with National Institute of Allergy and Infectious Diseases (NIAID).

To develop novel immune therapies for MDR-HIV or other virus-infected patients, LHRI has investigated anti-viral activity of multiple cytokine(s) and immune responses against virus infection, and discovered Interleukin (IL)-27 and IL-29 (Interferon-labmda1) as novel anti-HIV cytokines (Blood 2007, AIDS 2008, JICR 2010, PlosOne 2013, Nuc.Acid.Res 2013), novel micro RNAs as anti-viral/tumor RNAs (BBRC 2013, Sci.Rep 2018), Ku70 protein as a novel cytosolic DNA sensor that induces a novel innate immune response ( J.I. 2011, Sci.Signal 2017). LHRI has elucided SPTBN1 as a host restriction factor for HIV infection (JEM 2013) and a novel cell signal pathways via non-coding small RNAs (Nuc.Acid.Res 2014).

KEY ROLES/RESPONSIBILITIES

Postdoctoral Fellow will be involved in:
  • Development of immune therapy to inhibit MDR HIV
  • Identification of a protein in host cells that regulates not only HIV but also HIV-related virus infection and elucidate molecular mechanism of the regulation
  • Investigation of novel innate immune response.
  • Publishing paper(s) and presenting the findings in international or domestic scientific conference(s).

BASIC QUALIFICATIONS
  • Possession of a Doctorate degree from an accredited college or university according to the Council for Higher Education Accreditation. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency
  • No Experience required with a Doctorate degree
  • Must be able to obtain and maintain a security clearance.

PREFERRED QUALIFICATIONS
  • General molecular biology techniques (long RT-PCR, cloning of genes, genomic DNA isolation, construction of expression plasmids), tissue culture (cell lines and primary human cells: T cells, monocytes, NK cells, dendritic cells and macrophages), DNA, RNA or protein transfection, qRT-PCR, flow cytometer analysis using Flow Joe, western blotting, Immunol precipitation (pull down) assay, native or recombinant protein isolation using FPLC or HPLC, alpha screening, confocal microscopy, AMNIS, CRISPR technology, Protein mass and genechip assay.
  • Handling of replication competent HIV virus and lentivirus, NGS analysis, gel-shift (EMSA) assay.

Expected Competencies:
  • Strong background in immunology or HIV virology. The ability to challenge and establish new assay system(s).