Intern - Process Sciences

Location
Rensselaer, New York, US
Posted
Oct 02, 2018
Ref
14388BR
Required Education
Bachelors Degree
Position Type
Part time
Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

Project Description: This project will focus on the impact of test reagents and standards related to the Limulus amebocyte lysate (LAL) for endotoxin testing for Low Endotoxin Recoveries (LER).

Goal 1: Part 1 of this project will be an assessment of Beta (β) Glucans on LAL testing. β-Glucans are polysaccharides found in the cell walls of bacteria, fungi, yeast, and plants. This molecule can interact with the LAL reagent, which also reacts with endotoxin resulting in testing results that show higher endotoxin levels then are present in the sample. For this intern study, a deep dive into the sources of β-Glucans as well as alternative testing methods for endotoxin that selectively test for endotoxin will be explored. The goal of the study is to understand the impact of β-Glucans levels on endotoxin recoveries during various in-process steps.

Goal 2: The second part of this project will seek to understand the use of "natural occurring endotoxin" (NOE) on the LAL assay. During LAL testing, Control Standard Endotoxin (CSE) is used a reference standard for endotoxin and is comprised of purified lipopolysaccharide (LPS). However, endotoxin is generated from gram-negative bacteria and is comprised of the components of the outer membrane. NOE contains LPS along with surface proteins, lipoproteins and phospholipids and therefore could be more representative of the endotoxin found recovered in in-process samples. For this portion of the intern project, a spiking study to compare CSE and NOE will be performed. NOE will be generated from various common gram-negative bacteria found on site.

Goal 3: The final part of this project will combine the findings from the β-Glucans and NOE analysis to gain a better understanding of the LER found during endotoxin testing of in-process pools. The goal of this exercise will be to consider the conclusions from the previous experiments to greater characterize the current LAL assay and what potential continuous improvements could be implemented and how it may relate to LER.

Desired Majors and/or Skills:
- Microbiology, Biochemistry, Biotechnology.

Desired Education Level:
- Rising Soph., Rising Jr., Rising Sr.

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.