Research Associate III (NCI)

21701, Frederick
Sep 24, 2018
Science/R&D, Research
Required Education
Bachelors Degree
Position Type
Full time


The Clinical Pharmacodynamic Biomarkers Program is responsible for identification of biomarkers of response to new targeted agents entering clinical trials in the Developmental Therapeutics Program of the National Cancer Institute (NCI). The scope of work includes assay design, development, validation, fitness for purpose testing, transfer of assays to clinical laboratories, and clinical specimen analysis when required. PADIS facilitates mechanistic and performance analysis of putative anti-cancer agents, diagnostic tests, and therapeutic interventions to clinical applications, including distinct molecular signatures for cancer, refined molecular assays, and state-of-the-art imaging techniques that are too high-risk for industry or academia to pursue.


The Research Associate III will:

  • Develop and implement multiplex immunofluorescence assays using confocal microscopy or whole slide imaging in support of clinical studies and initiatives
  • Perform data analysis and present results from immunofluorescence assay studies on samples ranging from preclinical in vitro cell line experiments to fixed xenograft tissue sections to clinical specimen biopsies
  • Design, test, and write detailed technical standard operating procedures (SOPs) for imaging and analysis for transfer to external labs
  • Generate materials and data necessary to support the primary experimental objectives of the program
  • Design, perform, analyze, and present in vitro experiments to test pharmacodynamic marker responses to novel anti-cancer therapeutics in multiple formats, but particularly in multiplex immunofluorescence analysis of tissue based assays


To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

  • Possession of a Bachelor’s degree from an accredited university according to the Council for Higher Education Accreditation in a field related to biomedical research. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency.
  • In addition to the educational requirements, a minimum of eight (8) years of related work experience
  • Must be able to obtain and maintain a security clearance
  • Demonstrated experience in cancer biology or drug screening strategies is required
  • Outstanding English language and verbal communication skills are required. Ability to communicate with other researchers in a demanding scientific environment through presentations and preparation of formal reports is essential.
  • Ability to maintain precise records
  • Experience with quantitative image analysis software (e.g., Definiens)
  • Experience with fluorescence widefield and confocal microscopy
  • Ability to master novel technologies and instrumentation in response to changes in project priorities or breakthrough technological innovations; hands-on instrumentation work is required for this position
  • Ability to follow, create and write detailed technical SOPs for imaging and analysis


Candidates with these desired skills will be given preferential consideration:

  • Experience with tissue-based immunofluorescence assays.
  • Experience in the following areas: design and validation of multiplexed immunofluorescence assays; ability to work across testing platforms during the assay development process.
  • Statistical analysis methods and software
  • Design of cell-based assays to test mechanism of action of candidate compounds
  • Background in oncology
  • Familiarity with GLP requirements, HIPAA, and HiTECH regulations

Expected Competencies:

  • High content image analysis; Confocal Microscopy; Cancer Biology