Scientist/Sr. Scientist, Molecular Biology CAR-T Development
We are seeking an experienced Scientist with expertise in molecular immunology, immuno-oncology, and engineered cellular therapies to contribute to the development of allogeneic Chimeric Antigen Receptor (CAR) T cell therapies. The position will play a critical role in the generation and validation of new CAR-T products for clinical evaluation.
Prior hands-on experience in the design of CAR or TCR constructs and molecular characterization of immune cells is required. Investigation will focus on engineering and evaluating novel CARs using in vitro systems.
Reports to: Principal Scientist – CAR-T Development, Preclinical & Translational Science Location : Thousand Oaks, CA
Design and generate constructs for genetically modified allogeneic T cell products Independently designs and executes molecular CAR-T characterization workflows and IND-enabling studies to characterize and optimize expression and activity of CAR constructs Application of molecular workflows to synthesize and clone vectors using interbnal and external resources Collaborate with the CAR T platform development team to perform in vitro characterization of novel CAR- T cells in cell-based assays Designs and performs studies to examine molecular and biochemical mechanisms of action in vitro Establishes basis for novel product-specific assays, method development in collaboration with QC and analytical groups. Coordinates with external contract laboratories to generate novel reagents that compliment CAR products and facilitate product characterization and clinical correlative assays Provides shared support for ad hoc generation of research grade retroviral reagents, as needed Presents at group meetings and functions as SME for molecular biology in project team settings Opportunity to manage research associate(s)
Travel: Travel required (up to 10%)
Physical Requirements: Subject to extended periods of sitting and/or standing, vision to monitor and moderate noise levels. Work is generally performed in an office, laboratory, or manufacturing environment. Car and airplane (see above) travel is an essential part of the job
Professional Qualifications and Position Requirements:
PhD in Immunology and/or Cancer biology with >2 years postdoctoral (Scientist), >5 years postdoctoral experience (Senior Scientist) in T cell or immunotherapy-based research, including industry experience Experience with the design and synthesis of CAR expression constructs and evaluation of human CAR-T therapies; Industry experience in adoptive T cell, CAR-T, or TCR therapeutics strongly preferred In-depth understanding of T cell biology and/or CAR/TCR function within the tumor microenvironment and/or autoimmune contexts Technical expertise in primary immune cell isolation, immunophenotyping using flow cytometry, proliferation, and cytotoxicity assays Experience in gene editing technologies including CRISPR/Cas9 Working knowledge of all stages of CAR design and retrovirus generation (gamma or lentivirus) Significant contributions and productivity in immunology and/or cancer biology research as evidenced by publications in high impact journals and presentations at scientific conferences.