Postdoctoral Fellow (NCI)

Location
21701, Frederick
Posted
Sep 12, 2018
Ref
660782
Hotbed
BioCapital
Required Education
Doctorate/PHD/MD
Position Type
Full time

PROGRAM DESCRIPTION

LHRI - The Laboratory of Human Retrovirology and Immunoinformatics (LHRI), Clinical Services Program, Applied/ Developmental Directorate, has investigated mechanism multiple drug resistance (MDR) of HIV and innate immune response against HIV or HIV related virus infection collaborating with National Institute of Allergy and Infectious Diseases (NIAID).

To support developing novel immuno or chemo therapies for MDR-HIV infected patients.  LHRI has discovered that Interleukin-27 (IL-27) as a novel anti-viral cytokine and multiple novel micro RNAs as anti-viral RNAs. In addition, LHRI found a novel innate immune response/pathway against viral infection.

KEY ROLES/RESPONSIBILITIES

Postdoctoral Fellow will:

  • Investigate a molecular mechanism of anti-HIV property of IL-27 in CD4 T cells and Dendritic cells focusing on gene expression profiles in host cells and post translational modification of viral protein(s) utilizing virological and immunological techniques
  • Involve in studies of a role of IL-27 on immune cell function including innate immune response and ROS induction and investigate the function and mechanism of novel anti-viral micro RNAs induced by IL-27

Qualifications

BASIC QUALIFICATIONS

To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

  • Possession of a PhD or MD/PhD degree from an accredited college or university according to the Council for Higher Education Accreditation. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency
  • No experience is required beyond the Doctorate degree
  • Must be able to obtain and maintain a security clearance

PREFERRED QUALIFICATIONS

Candidates with these desired skills will be given preferential consideration:

  • General molecular biology techniques
    • Tissue culture (primary human cell and cell lines),
    • DNA, RNA or protein transfection,
    • qRT-PCR
    • Cloning and mutagenesis of gene of interest
    • Flow cytometer analysis using Flow Joe
    • Western blotting
    • Immuno precipitation/pull-down assay
    • Gel shift assay
    • Recombinant protein isolation using FPLC or HPLC
    • Alpha screening
    • Confocal microscopy
    • AMNIS and Fluorescence microscopy
    • Application of Protein mass and Genechip assays
    • Handling of replication competent virus (HIV and HSV) and incompetent lentivirus
    • Sanger sequence and NGS

0-3 years of experience in human immunology, virology or molecular biology