Associate Scientist, Protein Engineering

Location
Seattle, WA, United States
Posted
Aug 25, 2018
Ref
1150204
Required Education
Bachelors Degree
Position Type
Full time
Seattle, WA

Join bluebird bio's cutting-edge discovery research team developing novel technologies to enhance the success of our gene therapy and gene editing programs. The successful candidate will join our Protein Engineering team and provide critical support for ongoing and emerging research projects. Activities will include protein and construct design and cloning, DNA library preparation and screening, protein production and biochemical characterization, cell based assays, and high-content genomics assays such as next generation sequencing. Opportunities to grow into more advanced molecular engineering projects, sample generation techniques, and analytical assays are abundant.

About the position:
  • Creative protein design and optimization
  • Molecular cloning and plasmid production in e. coli and yeast
  • Gel electrophoresis and fragment analysis
  • Protein expression and purification
  • Lentiviral vector generation to genetically modify primary human cells
  • Immunological assays including ELISA and flow cytometry
  • Cell culture and aseptic technique
  • NGS library prep and analysis
  • Database entry and maintenance
  • Compile and present research data to functional teams
  • Provide lab support while working in a collaborative team environment

About You:
  • BS/MS degree in biology, cell biology, microbiology, immunology, biochemistry, bioengineering, biophysics or related discipline
  • 3+ year track record of lab experience with a protein biochemistry focus
  • Competency in basic molecular biology techniques, including primer design, PCR, agarose gel electrophoresis, bacterial transformation
  • Competency in protein purification techniques and biochemical analyses
  • Experience with mammalian cell culture and immunological assays
  • Experience with DNA software tools including with plasmid and sequence analysis (e.g. Geneious) and data analysis programs such as GraphPad Prism
  • Outstanding organizational and record keeping skills
  • Ability to meticulously maintain a lab notebook and contribute to support documentation
  • Be able to work in a highly collaborative team environment
  • Excellent communications skills and ability to influence across multiple functions
  • Willingness to embrace and demonstrate the core principles of the bluebird bio culture: b colorful, b cooperative, and b yourself