Scientist, Next Generation Sequencing Core (NCI)

21701, Frederick
Jul 26, 2018
Required Education
Position Type
Full time


The Cancer Research Technology Program (CRTP) develops and implements emerging technology, cancer biology expertise and research capabilities to accomplish NCI research objectives.  The CRTP is an outward-facing, multi-disciplinary hub purposed to enable the external cancer research community and provides dedicated support to NCI’s intramural Center for Cancer Research (CCR).  The dedicated units provide next generation sequencing, electron microscopy, protein characterization, protein expression, optical microscopy and genetics. These research efforts are an integral part of CCR at the Frederick National Laboratory for Cancer Research (FNLCR).  CRTP scientists also work collaboratively with intramural NCI investigators to provide research technologies and expertise.


The Sequencing Facility is a high throughput laboratory providing cutting edge next generation technologies to the National Cancer Institute.

The Scientist I will be responsible for:

  • Assisting with Next Generation (NGS) Sequencing for the core facility. Current platforms include the Illumina HiSeq, NextSeq, MiSeq, PacBio, and additional platforms in the future. All projects are of a highly specialized nature and require technical proficiency, problem solving skills and good judgment
  • Work with SF Research & Development group, Director, group leaders and informatics staff to develop, implement, and perform laboratory processes in support of the SF operations, especially for development in the field of long read technology
  • Assist in the identification, research, optimization and implementation of new processes or technologies that serve to introduce new applications, or improve data quality or efficiency of production pipelines, especially with a focus on long read sequencing applications
  • Coordinate with SF, quality management and informatics personnel to implement updates to equipment, chemistry, and processes
  • Remain current with new applications and technologies associated with NGS sample preparation, library construction, sample enrichment and target capture


To be considered for this position, you must minimally meet the knowledge, skills, and abilities listed below:

  • Possession of a Doctoral degree from an accredited college or university according to the Council for Higher Education Accreditation. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency.
  • No work experience beyond the doctorate is required for appointment at this level; however, some candidates may have had one or more years of postdoctoral research and training
  • Demonstrated experience in molecular biology or genetics/genomics, isolation, and manipulation of DNA and RNA, gel electrophoresis, high throughput platforms, applications, and technology
  • Demonstrated ability for independent design and performance of experiments, capable of trouble shooting assays or production problems, and ability to develop/optimize new assays or production methods under the direction of a lab supervisor or scientist
  • Proficiency in MS Excel, Word, and PowerPoint
  • Must be able to obtain and maintain a security clearance


  • Experience working within a core/service facility
  • Experience with Illumina (Solexa), Pacbio, or other NGS sequencing platforms, generation of libraries, sequencer operation, and maintenance
  • Experience with next gen sequencing development, troubleshooting, analysis, and laboratory automation
  • Familiarity with usage of a LIMS
  • Good oral and written communication skills, and proficient organizational and time management capabilities