Associate Scientist, T cell Gene Editing
Join bluebird bio's Cambridge-based genome editing team to help bring innovative immunotherapy products into the clinic. In this role, you will contribute to the preclinical and clinical development of gene-edited CAR/TCR T cells for oncology indications. An ideal candidate is a self-motivated, innovative scientist with a solid background in immunology (especially in T cell biology), and a strong interest in genome editing.
About the role:
About the role:
- Develop and optimize protocols for T cell gene editing, including: megaTAL delivery methods, culture conditions, scale up, and multiplexing
- Evaluate gene editing approaches to improve the efficacy of CAR and TCR based T cell therapeutics in vitro and in vivo
- Characterize genetically modified primary T lymphocytes using standard immunological assays including flow cytometry
- Maintain an electronic lab notebook and contribute to support documentation when necessary
- Contribute to experimental design and project direction
- Provide critical lab support while working in a collaborative team environment
- Compile and present research data to functional teams
- BS/MS in cell biology, immunology or related discipline and 1+ years of relevant research experience, (industry experience preferred)
- Extensive experience in mammalian cell culture, with cell culture of primary human T lymphocytes desired
- Experience with in vitro immunology studies and functional T cell assay development using techniques such as multi-color flow cytometry, Luminex, ELISA and cytotoxicity assays
- Experience in gene delivery into T cells or primary cells using mRNA transfection or lentiviral vector transduction preferred.
- Experience with standard molecular biology techniques, including but not limited to, PCR, RT-PCR, qPCR, Westerns, and RNA in vitro transcription preferred.
- Excellent computer skills, including experience with Microsoft Excel and PowerPoint, Graphpad Prism, DNA software tools (e.g. Geneious), and FlowJo
- Scientifically rigorous, highly organized, and with significant attention to detail
- Excellent oral and written communication skills
- Ability to work independently as well as collaborate with peers and effectively work in a results oriented research team environment
- "Do-what-it-takes" attitude
- Be ready to embrace the principles of the bluebird bio culture: b colorful, b cooperative, b yourself