Bristol-Myers Squibb Company

Senior Research Investigator

Location
Redwood City, CA, US
Posted
Jul 26, 2018
Ref
1R1503885
Required Education
Doctorate/PHD/MD
Position Type
Full time
Senior Research Investigator

Bristol-Myers Squibb is a global biopharmaceutical company whose mission is to discover, develop and deliver innovative medicines that help patients prevail over serious diseases.
One shared journey is moving us forward at Bristol-Myers Squibb. Around the world, we are passionate about making an impact on the lives of patients with serious disease. Empowered to apply our individual talents and ideas so that we can learn and grow together. And driven to make a difference, from innovative research to hands-on community support.
Bristol-Myers Squibb recognizes the importance of balance and flexibility in our work environment. We offer a wide variety of competitive benefits, services and programs that provide our employees the resources to pursue their goals, both at work and in their personal lives.

The Immuno-Oncology Resistance Biology group at Bristol-Myers Squibb in Redwood City, CA is seeking a highly motivated, interactive, and productive scientist to join a research group advancing novel early therapeutic targets centered on primary and acquired resistance to immune therapy of cancer. The position will utilize cellular and molecular biology to engineer and characterize cells expressing or
suppressing gene expression through recombinant methods. Engineered cells and cell lines will be profiled for validated expression as well as in vitro functionality of the target(s) through existing or newly-developed assays. The position will also entail building and operating a next generation sequencing lab as well as management over the design and generation of pooled CRISPR-Cas9 libraries for target identification screening efforts. The position will require the individual to be highly collaborative and work closely with and support an array of project teams and researchers within the Discovery Research organization.

Responsibilities:
• General molecular biology involving isolation, manipulation, and characterization of DNA, RNA and proteins
• Design of mammalian cell expression vectors for recombinant proteins, inhibitory RNAs, and CRISPRs
• Mammalian cell culture including hematopoietic cells and cell lines, including those of rodent or human origin
• Mammalian cell manipulation including transfection or lentivirus transduction of expression, interfering RNA, or CRISPR vectors
• Assessment of RNA and protein expression using qRT-PCR, flow cytometry, ELISA, HTRF or western blotting as appropriate
• Cell cloning and freezing
• Initial cell-based functional assay development and validation of newly engineered cells
• Detailed, formal record keeping through electronic notebook
• Communication of work, plans and progress using written and verbal communication in small and group settings

Qualifications
• PhD degree with 4+ years of relevant postdoctoral experience within the cellular/molecular field of practice. Experience in the field of immunology or oncology is ideal but not essential
• Demonstrated experience in design and construction of cell expression plasmid constructs, site-directed mutagenesis, and recombinant protein vector design
• Demonstrated experience in pooled or arrayed CRISPR library generation and screening, including downstream library preparation next gen sequencing is ideal.
• Extensive cell culture techniques including demonstrated ability to establish transient/stable cells and cell lines through transfections and/or virus transduction. Experience in work with primary human or rodent cells.
• Expertise in characterization of protein expression through biochemical (ELISA/Western) and cellular analysis (binding,reporter analysis) and RNA expression using RT-PCR. Flow cytometry experience is a plus.
• Demonstrated capabilities in cell-based in vitro assays and assay design and execution
• Ability to multitask among multiple projects and teams and work independently in a fast-paced, highly interactive environment
• Excellent oral and written communication skills