Molecular and Cellular Neuroimmunologist
Amgen Neuroscience is looking for a motivated cell and molecular biologist to be a driver of our efforts to develop therapies for neurodegenerative disease. As the field of neuroimmunology holds great potential for neurodegenerative disease treatments, the candidate will bring a perspective on the innate immune system to construct assays and uncover decision-driving human biology. This position is for the dedicated experimentalist who will work in a multidisciplinary team as part of our pre-clinical research. Core areas of focus will be translating genetics and human biology observations into both in vitro and in vivo systems to understand modifiers of neurodegenerative pathology. Also, this position will be charged with exploring the emerging target space of the immune system in the brain via functional genomics (siRNA, shRNA, CRISPR/Cas9, and chemogenomic screens), proteomics, and transcriptomics.
A second point of emphasis for this position is innovation in genome editing techniques (CRISPR/Cas9 and variants) to advance neurodegenerative disease research. To validate targets in the neuroimmunology space, we seek to integrating cell line generation, molecular biology, and genome editing methodologies. Working effectively with bio-informaticists, chemists, and a variety of in vivo and in vitro-oriented collaborators is essential for success. This position is designed for someone seeking to become a technical leader and a critical resource within Amgen Neuroscience. A core aim will be discovering new biological insight to enable drug discovery programs within Amgen and to impact the external scientific community through publication.
- Planning and execution of molecular and cellular experiments to understand fundamental neurological and immunological processes. Responsible for delivering reproducible and well-controlled datasets according to project timelines.
- Generation of in vitro models and assays for drug discovery and target validation to demonstrate that these assays are robust and appropriate for large-scale screening campaigns.
- Develop deep understanding of neurodegenerative disease biology in the context of Amgen Neuroscience priorities using appropriate experimental and analytical approaches.
- Assist team members in integrative experiments that measure the response of in vitro and in vivo systems to pharmacological modulation.
- Contributedatasets for project teams in addition to observations, innovations, ideas, and literature insights into the development of novel approaches to study the immune processes in the brain.
- Doctorate degree or Doctorate degree to be completed by January 2019
- Master's degree and 4 years of bioscience experience
- Bachelor's degree and 6 years ofbioscience experience
- Deep expertise in cellular and molecular biology techniques including and the latest methodologies in vector construction, site-directed mutagenesis, qRT-PCR, DNA/RNA/Protein visualization, labeling, and RNAi
- Demonstrated application of mammalian cell culture and associated techniques with an emphasis on cell line development
- Previous research focused on the innate immune system
- Experience with fluorescence activated cell sorting (FACS)
- Ability to design and utilize viral-based gene transduction systems
- Strong proficiency in written and spoken English with the ability to convey ideas and information clearly
- Ability/willingness to work with in vivo models such as rodent
- Highly proficient with statistics, figure design, and scientific data analysis software (e.g., Graphpad Prism, Image J, Adobe Photoshop/Illustrator, SigmaPlot, Metamorph, Origin, and R)
- Successful utilization of genome editing techniques such as CRISPR/Cas9 and TALEN platforms
- Pharmaceutical industry experience