Research Technologist- Stem Cell Core
The Gladstone Institutes is an independent, not-for-profit research institution affiliated with the University of California San Francisco (UCSF), transforming the health and well-being of all people through medical research, education, and outreach in the fields focused on cardiovascular biology, immunology, neuroscience and stem cell biology to overcome unsolved diseases. Home to notable leaders in the field of medical science, including 2012 Nobel Prize in Physiology or Medicine recipient Shinya Yamanaka, M.D.,Ph.D. Gladstone is comprised of four institutes in addition to various Centers and programs focusing approximately 500 members of our team on its mission: Science Overcoming Disease. Located in an award-winning building adjacent to UCSF's Mission Bay Campus, Gladstone offers a competitive salary and benefits program within a robust environment for those seeking to maximize their potential. Please consider Gladstone and make a difference in human health.
We are currently recruiting for a Research Technologist I position in the Gladstone Stem Cell Core (GSCC)
Cell Culture & Laboratory Duties
•Culture, expand, catalogue and disseminate cell lines including human induced pluripotent cells (iPSC), fibroblasts, and peripheral blood mononuclear cells (PBMCs).
•Perform human iPSC reprogramming from fibroblasts and PBMCs.
•Differentiate human iPSC lines into cardiac and other lineages.
•Perform quarterly mycoplasma testing on all GSCC-cultured cell lines and report results effectively to user.
•Perform standard molecular biology techniques such as DNA extractions, PCR, gel electrophoresis, transfections to support genome editing in human iPSCs.
•Perform FACS analyses, immunocytochemistry, microscope imaging and other techniques.
Operational & Lab Management Duties
•Work collaboratively and cohesively in a small team.
•Report to core Director regularly and attend stem cell core lab meetings.
Manage and operate core facility semi-independently.
•Interact and communicate effectively with multinational researchers on a weekly basis.
•Provide stem cell technique training expertise to both junior and senior researchers.
•Enforce core rules, procedures and guidelines.
•Communicate with vendor representatives to maintain professional relationships and negotiate equipment/material/service discounted pricing.
•Stay informed about stem cell related products and services; disseminate product/service literature to the Gladstone stem cell community; arrange for sample testing and equipment demonstrations with vendors.
•Readily answer researchers' questions with expert biological and technical advice; problem-solve with feasible solutions; exercise resourcefulness, creativity and proactivity to seek answers/solutions from other sources when encountering problems outside range of expertise.
•Work with department lab managers and lab aides to coordinate common operational procedures.
•Serve as a primary contact for equipment malfunctions/repairs; report necessary equipment repairs to our Facilities team; follow through to ensure repair or replacement.
•Have working knowledge of general laboratory equipment functionality and troubleshoot problems.
•Become familiar with core's online ordering system; compile monthly recharges for accounting.
•Manage time appropriately in an environment requiring attention to numerous activities and shifting demands.
•Accept constructive criticism and implement operational modifications where necessary to improve accuracy, efficiency, and cost-effectiveness.
•Minimum: B.S. in a field of Biological Sciences
•1-2 years' experience in a laboratory setting applying human stem cell culture techniques is desired
•Certification in Stem Cell Science is a plus
•Experience with human iPSC derivation is preferred
•Experience working with a team of scientists to provide research support and laboratory management is preferred
•Must be available for limited work on most weekend
The ideal candidate will be personally motivated to facilitate cutting-edge disease-related research in a modern scientific facility using their training in pluripotent stem cell culture, and employ their own desire to provide order to the sometimes-chaotic environment of a busy research laboratory. The objective of the person in this position will be to help other investigators attain ambitious research goals by providing support services and an organized environment where resources can be most efficiently, effectively accessed and utilized by a group of 50 researchers.
Title and salary will be commensurate with education and experience.