Scientist - Bioassay Development and Cell Line Engineering

Location
Tarrytown, New York, US
Posted
May 19, 2018
Ref
13077BR
Required Education
Doctorate/PHD/MD
Position Type
Full time
Position Summary

Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

Summary
The Bioassay group in the Therapeutic Proteins Department seeks a highly motivated scientist to join a fast-paced, dynamic group involved assay development and high-throughput screening in early drug development. The candidate will design and develop bioassays for screening and characterization of antibodies for various therapeutic areas and lead efforts in cell line engineering projects for in vitro and in vivo assays.

This person will develop cell lines and assays, perform high-throughput screens and characterize lead candidate antibodies in support of the company's Velocimmune antibody pipeline. Other responsibilities include, but are not limited to:

• Lead and contribute to assay development efforts by designing, performing and optimizing cell-based assays (e.g. luciferase, Ca++ flux, viability assays, etc.), enzymatic and ELISA-based assays, and label-free detection methods, imaging-based assays, etc.
• Generate and characterize cell lines: maintenance of cells, transient transfection, virus transduction, stable cell line generation, single-cell cloning, flow cytometry and immunocytochemistry, immunoblotting, high content screening
• Design and generate knock-out and knock-in cell lines with genome engineering. Characterize modified cell lines by PCR, sequencing, qPCR, NGS, and other techniques.
• Design and execute high-throughput screens using 96-and 384 well formats.
• Analyze data, trouble-shoot experiments, meet timelines, and prepare summary slide presentations.
• Keep up with advances in technology and relevant disease biology.
• Communicate results to supervisor and senior managements effectively.
• Record experiments accurately in a timely manner.
• Prepare reports for patents and INDs.

Requirements
This position requires a minimum of a Ph.D. degree in cell biology or a related field, with 0 - 4 years postdoctoral experience. Experience should include:
Required: In-depth knowledge of signal transduction pathways, mammalian cell culture/cell line generation, immunocytochemistry and immunoblotting, molecular biological techniques

Highly preferred: Genome editing by CRISPR/CAS9

Preferred: Multi-well assays, cell-based assays, ELISA, flow cytometry

Level will be commensurate with experience

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.

Requisition Number: 13077BR