Immune Cell Biology Research Associate

Location
La Jolla, California
Posted
Apr 30, 2018
Required Education
Bachelors Degree
Position Type
Full time

The qualified candidate will be working with the team developing new assays that will support the functional profiling and safety evaluation of Synthorx therapeutic agents. He/she will implement a variety of cellular assays to monitor the in vitro and ex vivo pharmacology of large molecule (biologic) leads at T and natural killer (NK) cell subsets. The candidate will also play a key role in the design and implementation of read-outs for T cell function and immune toxicity (cytokine release, vascular leakage), including samples from in vivo studies. He/she will actively scout the pre-clinical and clinical literature, and patent records, in search for example molecules and study systems to test the pharmacological and safety properties of current and future targets in the Synthorx portfolio.

Responsibilities

  • Implement complex cellular assays (flow cytometry, lymphocyte quantification, T cell stimulation, etc.)
  • Assay of Cytokines in plasma samples and summarize data
  • Culture and maintain cell lines and primary cells, for cell-based assays. Extensive experience with immune cells is a must
  • Proficiency in the isolation and characterization of blood cell subpopulations employing cytometry and additional methods for immune cell analysis
  • Manage mammalian cell stock inventory
  • Coordinate with other lab members day-to-day lab activities including equipment maintenance, reagent orders, restocking consumables, etc.
  • Develop appropriate assays to support analysis of large molecule pharmacology in in vivo models
  • Effective work and communication with group members and contract research organizations, in a dynamic and fast paced environment to accomplish goals
  • Preparation and presentation of power point summary data slides

Requirements

  • BS or MS in biomedical sciences
  • 2+ years lab experience in Immunology or related field
  • Experience with cell culture and aseptic technique is required
  • Experience in ELISA and/or MSD is required
  • Experience with multiparametric flow cytometry is required
  • Experience with handling human tissue specimens (mostly blood) is a requirement, including the isolation of human PBMC and other subpopulations
  • Experience with cell sorting is a plus
  • Experience with animal models is a plus
  • Familiarity with BSL-2 protocols and best-practices is a plus
  • Experience with tumor-dissociation and analysis of tumor infiltrating cells is a plus