Proteomics and Biomarkers Intern, Summer 2018

Location
Tarrytown, New York
Posted
Apr 19, 2018
Ref
11154BR
Discipline
Science/R&D, Research
Required Education
Bachelors Degree
Position Type
Part time

Position Summary

Known for its scientific and operational excellence, Regeneron is a leading science-based biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions. Regeneron commercializes medicines for eye diseases, high LDL-cholesterol, atopic dermatitis and a rare inflammatory condition and has product candidates in development in other areas of high unmet medical need, including rheumatoid arthritis, asthma, pain, cancer and infectious diseases.

These positions are in Proteomics and Biomarkers in Tarrytown, NY. There are 4 openings for Summer 2018. Each opening is described below.

Position 1: The student will develop a cell bank of primary human cells for proteomic cell line characterizations, isolate and expand different primary human cell lines for downstream proteomic and ncADC experiments and investigation. The student will also matrix and cell line controls and standards. Characterization will include characterization of isolated cells via FACS and access labeling efficiency using mass spec for SILAC labeled cell types.

Position 2: The student will develop mass spectrometry based workflows to support antibody drug discovery and development.

Position 3: The student will be responsible for learning a variety of proteomics approaches to support projects of Therapeutic Focus Areas (TFA) and Therapeutic Proteins (TP).
Human leukocyte antigens (HLAs) present a repertoire of endogenously derived peptides for immune surveillance by T lymphocytes. Recently it has been reported that a variety of cancers present abnormal post-translational modifications (PTMs) of HLA peptides which may alter the normal antigen processing pathways, resulting in the display of these modified peptides by HLA molecules on the surface of tumor cells. Different PTMs might also be induced by various mechanisms including inflammation, cellular transformation, death and aging. Here we developed a Mass spectrometry-based discovery workflow to identify HLA presented peptides in tumor samples. We have been profiling unmodified immunopeptidome from HLAs, and now are interested in mining this data for PTMs. The goal of this project requires data mining of mass spectrometry data to identify novel post translationally modified peptides derived from tumor antigens. Abnormal post-translational modification peptides could present new repertoire of targets for immunotherapies. We are seeking a results-driven Intern who is energetic, enthusiastic, and enjoys collaborative science research to join Bioanalytical & Biomarker Technologies.

Position 4: The student will be working in a state-of-the-art mass spectrometry lab, and responsible for learning a variety of proteomics approaches and techniques, to support projects of Therapeutic Focus Areas (TFAs) and Therapeutic Proteins (TP). With the continued growing interest and requirement for better understanding of various cell types and their surface protein kinetics/turnover this is a unique project. The overall goal of the student will be to develop and establish an in-house proteomic cell bank of primary human cells for proteomic investigation and assist in the characterizations of lead Regeneron molecules. This will involve the isolation and expansion of different primary human cell lines, as well as phenotypic characterization, via cytokine profiling and flow cytometry for downstream proteomic and ncADC scientific investigation. Responsibilities and experiences will include, but not limited to; aseptic in vitro cell culture, isolation and expansion of primary human cells, cell characterization via flow cytometry, in vitro metabolic labeling and mass spectrometry. This cell bank will establish a robust and comprehensive reserve of different primary human cell for downstream scientific investigation into lead therapeutic molecules. Techniques will include handling of primary human cell lines, characterization of isolated cells via FACS and assessment of in vitro metabolic labeling efficiency using mass spectrometry. With a variety of methods and techniques being applied, an ability to learn and multi-task is critical. The student's exposure to Regeneron's cutting edge proteomic techniques and collaborative efforts, make this a great opportunity. Ultimately the results the project work will lead to critical support of TFA and other technology development programs within all Regeneron.

Start building your skills at Regeneron.
• Must be an undergraduate student
• Must be pursuing a degree in Biology, Biochemistry, or Chemistry
• Must have a basic understanding of in-vitro and proteomics techniques (cell culture, flow cytometry, western blotting, light microscopy, etc.)
• Knowledge in Immunology and Mass spectrometry is preferred, but not required
• Bioinformatics a plus for position 3
• Must have basic computer skills and operation of Microsoft Office applications. Must have the ability and drive to learn new technologies fast with good problem-solving skills. Must have overall enthusiasm in science with motivation to contribute to a larger team environment. Must have excellent communication skills.
• For position 2: 3rd or higher year of PhD in Analytical Chemistry, experience with mass spectrometry instrumentation/application, Must have demonstrated publication record in peer reviewed journals, Must have hands-on experience with LC-MS instrumentation and applications, Experience with native MS technology for intact-MS analysis, HDX-MS for protein conformation analysis, and automation for sample preparation a plus.

General Intern Program Information:
• Must be enrolled in, or accepted to, an academic program pursuing a Bachelor's, Master's, PhD or PharmD
• Summer Program is full-time (~40 hrs/wk) for at least 10 weeks and is paid
• Prefer demonstrated leadership in areas such as campus activities, clubs, sports or the community
• Minimum GPA of 3.0
• You will work with a specific manager on a project(s)
• Enjoy weekly 1 hour Intern Program events: learn about other areas of the company from VPs and other employees, soft skills workshops, networking, volunteering, and more!
• Present to your team on your summer project

Transportation and Housing:
• A free shuttle is offered from the North White Plains or Tarrytown train stations to the Tarrytown campus
• Get to know other incoming Interns on our closed LinkedIn page when you accept an offer

This is an opportunity to join our select team that is already leading the way in the Pharmaceutical/Biotech industry. Apply today and learn more about Regeneron's unwavering commitment to combining good science & good business.

To all agencies: Please, no phone calls or emails to any employee of Regeneron about this opening. All resumes submitted by search firms/employment agencies to any employee at Regeneron via-email, the internet or in any form and/or method will be deemed the sole property of Regeneron, unless such search firms/employment agencies were engaged by Regeneron for this position and a valid agreement with Regeneron is in place. In the event a candidate who was submitted outside of the Regeneron agency engagement process is hired, no fee or payment of any kind will be paid.

Regeneron is an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, sexual orientation, gender identity, disability status, protected veteran status, or any other characteristic protected by law.

Requisition Number: 11154BR