Sr. RA / Scientist - Gene Editing
Sr. RA / Scientist – Gene Editing
The ideal candidate should be experienced the latest molecular cloning and sequencing techniques for the generation and analysis of transgenic T cells. The candidate should enjoy operating in an exceptionally dynamic and cooperative environment and will communicate results to and coordinate efforts with a larger cross-disciplinary team of immunologists, biologists, engineers and bio-informaticians.
- The candidate will be performing experiments to validate and improve the technology for gene editing (knock-in and knock-out) of endogenous and neoE TCRs, and analyzing the resulting data.
- She/he must be able to communicate clearly and concisely, both verbally and in the form of written reports. The incumbent will aid in drafting protocols, and provide data for regulatory documentation to support clinical development.
- The ideal candidate will hold a Master’s degree with 3+ years’ experience, or a recent PhD; with career focus in cell or molecular biology, biochemistry, or molecular genetics.
- Industry experience is strongly desired.
- The candidate must have hands on experience with nucleic acid purification and use, including primer design, PCR and standard cloning.
- The candidate should have experience in mammalian cell culture.
- The candidate should be highly goal-driven and have demonstrated the ability to focus on time-sensitive objectives.
An incumbent with experience in the following areas is a plus:
- High-throughput sequencing sample preparation and analysis
- qPCR and/or ddPCR assay design, execution, for analysis of gene-edited cells
- Large-scale plasmid DNA purification
- Mammalian cell transfection
- Primary T cell culture
- Eukaryotic protein expression systems
- Flow cytometry or FACS
- Site-specific nucleases (e.g. CRISPR and TALENs) and resulting cellular and molecular analysis