Research Associate I

Location
San Francisco, California
Posted
Apr 07, 2018
Ref
101936002
Required Education
Bachelors Degree
Position Type
Full time

The Gladstone Institutes is an independent, not-for-profit research institution affiliated with the University of California San Francisco (UCSF), contributing to the health and well-being of all people through medical research, education, and outreach in the areas of heart disease, HIV/AIDS, and neurological disease. Our employees receive exceptional benefits. We are located in an award-winning building adjacent to UCSF's Mission Bay Campus.

We are currently seeking to hire a Research Associate I. The Research Assocaite I will work as a member of the Finkbeiner lab to investigate mechanisms of neurodegenerative disease, in particular Parkinson's disease, using iPSC based models of disease and state of the art longitudinal robotic microscopy. The ideal candidate is someone who is passionate about science, can work both independently and as part of a team, and is eager to learn a variety of laboratory techniques.

Responsibilities:

  • Growing and differentiating iPSCs from patients with neurodegenerative disease
  • Oversee many iPSC lines and differentiating cultures
  • Perform experiments to validate the pluripotency and quality of iPSCs
  • Perform automated and manual imaging of differentiated neurons
  • Analyzing imaging data, including the manual curation of neurons
  • Learning and performing a variety of other lab techniques as needed to assist with projects
  • Helping with general lab upkeep, including aliquoting reagents, making media, and helping to keep the lab organized
  • Maintaining a thorough lab notebook and sharing results in a readable format as requested
  • Purifying plasmids and expressing constructs in neurons

This person will be expected to work independently as well as with others, clearly communicate progress to direct supervisor, and present progress during lab meetings.



Education Required:

  • B.S./B.A. degree required (Molecular Biology, Cell Biology, or related field) and 1-2 years of related work experience.

Skills/experience required:

  • Sterile technique
  • Mammalian cell culture

Experience preferred:

  • Immunocytochemistry
  • Fluorescence microscopy
  • RNA isolation, plasmid miniprep, genomic DNA isolation, DNA gel
  • Cell line maintenance, including: cell splitting, cell banking, cell freezing, cell thawing, iPSC picking, iPSC cleaning
  • Cell lysate collection
  • Cell transfection

PI101936002