Research Associate/Senior Research Associate, Protein Production Process Development

La Jolla, California
Mar 02, 2018
Required Education
Bachelors Degree
Position Type
Full time

Synthorx is a pharmaceuticals startup company using a novel synthetic biology platform for the discovery and production of improved protein therapeutics.  We have advanced a revolutionary technology that expands the genetic alphabet (A, T, G, C, and X, Y), to drive the site-specific incorporation of multiple non-natural amino acids into proteins of any size, from small peptides to large biologics, and to manufacture these improved proteins at scale. This technology enables the design of biotherapeutics with useful chemical functionality and opens-up opportunities to make novel protein therapeutics to previously undruggable targets. We are hiring a Research Associate/Senior Research Associate to join the Production group.  The successful candidate will have a strong background in protein expression in E. coli and a working knowledge of protein biochemistry and purification.

Key Responsibilities:

  • Perform all steps of protein expression and purification, analytical and scale-up. Experience with fermenters is a plus
  • Experimentally determine methods to optimize yields for protein expression
  • Establish methods for refolding and purification of expressed proteins
  • Experience with production of peptides employing recombinant systems
  • Develop standard operating procedures, document protocols and technology transfer
  • Prepare and present project updates to ensure timely delivery
  • Maintain a high standard for quality control including up-to-date data records for all protein deliveries, including basic understanding of mass spectrometry methods for QC
  • Troubleshoot challenging technical, experimental, and instrumental issues
  • Consummate ability to work in a highly collaborative, fast-paced, cross-functional environment, with superior oral and written communication skills


  • BS or MSc degree, with a major in biochemistry, biology or related field, and 3+ years of hands-on experience with recombinant protein production
  • Experience with E. coli-based protein expression. Scale-up preparation employing fermentation instrumentation and protocols is a plus
  • Experience developing methods for protein purification. Familiarity with protocols involving extraction from inclusion bodies and refolding is a plus
  • Chromatography by affinity, IEX, and SEC. Familiarity with AKTA instrumentation is highly desirable
  • Experience in protein characterization (SDS PAGE, Western Blotting, quantification). Understanding of MS methods for QC is a plus
  • Hands-on familiarity with standard molecular biology methods
  • Regular activities require ability to communicate effectively and rapidly reprioritize to match dynamic and demanding timelines

Additional preferred skills:

  • Basic understanding of cancer and/or immuno-biology