The He Research Group at the Fischell Department of Bioengineering, UMD, uses Linkam temperature- controlled microscopy to study multiscale biomaterials to engineer living cells and tissues for cancer treatment and cell-based medicine.

Tadworth, UK- 8th August 2018: Market leaders in temperature-controlled microscopy and leaders in sample characterization solutions, Linkam Scientific Instruments, reports on the research of Professor Xiaoming “Shawn” He of the Fischell Department of Bioengineering at the University of Maryland. Professor He's research is focused on developing multiscale biomaterials to engineer living cells and tissues for two applications: Cancer treatment and cell-based medicine.

Dr Xiaoming “Shawn” He is a newly appointed Professor in the Fischell Department of Bioengineering at the University of Maryland. He leads a research group that is focused on developing multiscale biomaterials to engineer living cells and tissues for two applications: Cancer treatment and cell-based medicine. His base is the Multiscale Biomaterials Engineering Laboratory. It is dedicated to research and education on developing multiscale (nano, micro and macro) biomaterials and devices with bioinspired spatiotemporal complexity to (1) encapsulate and deliver small molecules, genes, peptides/proteins, cells and tissues and (2) engineer 3D biomimetic systems in vitro with the ultimate goal of improving the safety and efficacy of cancer treatment, tissue regeneration and assisted reproduction.

One of the important fields in this work is lyophilization, the study of freeze drying. Professor He's Group studies ice formation during cooling and freezing biological samples to minimize the damage to biological cells during cooling and freeze-drying biological samples for banking at both cryogenic and ambient temperatures.

Over his research career starting in the Department of Biomedical Engineering at Ohio State University, Professor He has always selected Linkam stages for his freeze drying studies. This originated from the fact that Linkam stages have been reported in many published studies allowing him to compare his work with that in the literature. Professor He has been using the Linkam FDCS196 stage in his research to study ice formation. The FDCS stage is widely used in lyophilization laboratories across the world. It combines a cryo stage with light microscopy techniques to quickly and accurately determine collapse and eutectic temperatures and intricately investigate the freeze dried structure of complex samples.

Professor He has published widely. These include a paper which describes new ways of cryopreservation of stem cells.1 This is important to help meet the ever-increasing demands of cell-based medicine. The role of the Linkam stage was to study the inhibition of devitrification and intracellular ice formation. A second paper,2, looks at ways of stopping cell injury that may occur in the cooling and warming processes. Here, the FDCS stage was used to mimic the ice seeding process and checking cell viability in situ.

For information on Linkam's range of temperature-controlled stages along with other temperature and environmental materials characterization solutions, click here to visit the web site.

1 Alginate Hydrogel Microencapsulation Inhibits Devitrification and Enables Large-Volume Low-CPA Cell Vitrification, Haishui Huang et al, Adv. Funct. Mater. 2015, 25, 6839–6850, DOI: 10.1002/adfm.201503047

2 Predehydration and Ice Seeding in the Presence of Trehalose Enable Cell Cryopreservation, Haishui Huang et al, ACS Biomater. Sci. Eng. 2017, 3, 1758−1768 DOI: 10.1021/acsbiomaterials.7b00201

About Linkam Scientific Instruments
Linkam develops and manufactures a broad range of temperature-controlled stages from high to cryo temperatures for both OEM and end users. These are used in conjunction with light microscopes and a wide range of analytical techniques including Raman, FTIR, WAX/SAX and other X-ray techniques to visualise and characterise the properties of materials. Linkam stages are found in thousands of laboratories worldwide with the most successful microscope heating stage, the THMS600, selling over 5,000 units alone. Linkam is the market leader in temperature-controlled microscopy.

About McCrone Microscopes & Accessories
McCrone Microscopes & Accessories, LLC is the authorized dealer for Nikon light microscopes, Linkam heating/cooling stages, the JEOL NeoScope benchtop SEM, HiROX Digital Microscope Systems, digital imaging systems, and a variety of laboratory supplies. The technical sales representatives at McCrone Microscopes are trusted advisors to microscopists worldwide.

Hooke College of Applied Sciences, LLC provides education and training to scientists worldwide. In addition to undergraduate programs offered in collaboration with North Central College, Concordia University Chicago, and Lewis University, Hooke College of Applied Sciences has more than 40 specialized short-courses in materials analysis. Topics covered include light and electron microscopy, spectroscopy, sample preparation, chemistry and laboratory safety, and image analysis.


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