ZyGEM Corporation and USAMRIID Sign Crada for Development of Simplified Sample Processing Solutions for Biothreat Agents
Published: Jan 05, 2012
The CRADA effort will leverage ZyGEM’s proprietary technology for the extraction of DNA and other nucleic acids from diverse samples using thermophilic enzymes produced by extremophile organisms. USAMRIID and ZyGEM intend to collaboratively explore the utility of a number of enzymes capable of isolating nucleic acids. ZyGEM’s enzyme technologies will be evaluated and optimized to provide sample processing capabilities for the detection of a variety of biothreat agents and for use in challenging environments.
ZyGEM currently markets products based on a similar approach. The unique properties of its EA1 protease, the key ingredient in the company’s prepGEM™, forensicGEM™, livestockGEM™ and RNAGEM™ families of nucleic acid extraction kits, make possible cost effective and flexible solutions for researchers performing a wide variety of studies, including human genotyping, animal testing and basic research. This enzyme has characteristics that make it well-suited for DNA and RNA extraction in a closed tube, where a simple temperature shift modulating enzyme activity rapidly provides high quality DNA and RNA that is ready for analysis by most PCR-based methods, while avoiding the contamination and low yields that can be encountered with other approaches.
“Molecular diagnostics offers the potential to increase the speed, efficiency and accuracy of pathogen detection, but its use has been limited by the fact that isolation of the nucleic acids needed for the analysis is often laborious and inefficient, yielding low quality material that is contingent on the target organism and the sample matrix,” noted David Saul, Ph.D., senior scientist at ZyGEM. “We believe our enzymatic approaches have the potential to enable the rapid preparation of high quality DNA and RNA for the timely detection of biothreat pathogens from a variety of sample matrices simply and efficiently, which could be especially valuable for use in demanding conditions in the field. We are very pleased to be working with the infectious disease experts at USAMRIID to assess and develop new approaches that seek to harness our unique enzymes to help combat potential biothreats.”
Established in 1969, USAMRIID’s mission is to conduct basic and applied research on biological threats resulting in medical solutions – vaccines, drugs and diagnostics – to protect our Nation’s armed forces. Its research often has applications that benefit society as a whole. As the lead military medical research laboratory for the Defense Threat Reduction Agency’s Joint Science and Technology Office for Chemical and Biological Defense, USAMRIID plays a key role in national defense and in infectious disease research. USAMRIID, located at Fort Detrick, Maryland, is a subordinate laboratory of the U.S. Army Medical Research and Materiel Command.
ZyGEM Corporation markets nucleic acid extraction solutions based on the company’s exclusive collection of microorganisms from extreme environments, with applications in basic research, forensics, clinical diagnostics and agriculture. ZyGEM’s MicroLab unit is developing integrated sample-to-answer systems with the potential to revolutionize DNA testing. For more information about ZyGEM and its prepGEM™, forensicGEM™, livestockGEM™ and RNAGEM™ nucleic acid extraction kits, visit www.zygem.com.