New Harvard Apparatus Fluorescence-Based Detection of pH, CO2 and O2
Published: Sep 08, 2011
- More accurate measurements than traditional methods
- Sensitive sensors are also rugged for long life
- No electrolytes required
- Completely sterilizable sensors
- USP Class VI-certified sensors are shipped pre-calibrated
- Minimal chemical degradation means usage life and infrequent recalibration
In contrast to traditional ion-sensitive chemosensors, these optical biosensors use a controlled optical source that emits light signals at specified frequencies which excite an ion-specific sensor spot in contact with the test medium.
Changes in the parameter of interest are reported as a proportional change in some characteristic (intensity, phase shift, etc.) of the fluorescence. Because they are inherently self-referencing, this detection technology eliminates the potential for measurement error arising from changes in sample properties (i.e. flow rate, viscosity, etc.) over the duration of an experiment. This ensures stable, drift-free calibration and hence reliable measurements throughout the lifespan of a sensing element.
Unlike polarographic oxygen electrodes, this technology is non ion-consumptive. These easy-to-use biosensors are free of the maintenance complications inherent in traditional chemosensing: requiring only cleaning and dry storage, there is no need for electrolyte, membrane changes, or pre-polarization of the electrode amplifier.
For more information contact:
Stephanie Pazniokas, M.S.
84 October Hill Road
Holliston, Massachusetts 01746 USA
Toll Free: 800-272-2775
Harvard Apparatus is a global developer, manufacturer and distributor of innovative and specialized products to enhance bioresearch.