Link Technologies Ltd Release: New Cyanine Dyes Reduce Time and Cost of Oligo Labelling
Published: Mar 20, 2013
Until now, 3’-cyanine modification of an oligonucleotide could only be done post-synthetically, normally to an amino-modified oligo, or by the use of a cyanine phosphoramidite onto a universal or modified support that will not interfere with the intended use of the oligo. With Link Technologies’ innovative 3’-modified 1000Å CPG supports, customers can add cyanine dyes directly to their oligos of interest without any additional modification step. Link Technologies’ cyanine dye supports therefore save time by reducing the number of experimental steps and avoid the unnecessary cost of amino-modified oligos or the inconvenience of combining cyanine phosphoramidites and universal supports.
Used as fluorescent markers in oligonucleotide synthesis, dyes are central to numerous detection techniques, such as probes for monitoring real-time PCR, fluorescence in situ hybridisation, SERRS-based DNA detection assays and FRET studies in general (e.g. DNA-protein interaction studies). 3’-Labelling is particularly useful in the latter where the FRET partner is incorporated either at the 5’-end or within the oligonucleotide sequence.
For more information on the new Cyanine 540 and Cyanine 650 phosphoramidites and supports, and other oligonucleotide reagents, please visit the Link Technologies website at www.linktech.co.uk or follow us on Twitter: @linktechdna.
About Link Technologies Ltd
Link Technologies Ltd is a leading kilo-scale supplier of speciality reagents for oligonucleotide synthesis and modification, all to the ISO 9001:2008 certification. The company’s main markets are university and commercial research departments, plus, increasingly, larger Contract Manufacturing Organisations (CMOs) in the biotech sector.
Link has also forged successful links to academia working closely with many UK universities. In tandem with internal research and development, the Company has an excellent record in bringing in-licence products to market with various worldwide commercial partners.
For further information see: www.linktech.co.uk.