Integrated DNA Technologies Release: Not A Constant Value: Understanding Tm
Published: Dec 16, 2013
CORALVILLE, IA – 16 December 2013 – In the latest issue of DECODED, the quarterly newsletter form Integrated DNA Technologies, resident expert on nucleic acid thermodynamics, Dr Richard Owczarzy introduces researchers to a new approach for calculating nucleic acid melting temperature (Tm). Exploring the impact of environmental conditions on Tm, the article explains how researchers can use these calculations to enhance the performance of their hybridization-based protocols.
Oligonucleotide hybridization forms the basis of many molecular biology techniques, from PCR and NGS through to in situ hybridization and gene knockdown studies, and knowing the exact Tm is vital when designing these experiments. Dr Owczarzy explains how many researchers simplify their calculations of Tm as a fixed characteristic based on sequence composition. However, Tm is also dependent on experimental conditions, including oligo concentration, the ionic environment and Mg2+ availability, as well as sequence mismatches and SNPs. IDT has therefore developed a free online Tm calculator, delivering the most accurate calculations of Tm.
For more information or to download the latest issue of DECODED, please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the world, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; Leuven, Belgium; and Singapore. For more information visit www.idtdna.com.
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