Harvard Apparatus Release: Ready to Use Disposable 96-Sample Equilibrium Dialysis Plates

Published: Nov 29, 2011

November 29, 2011 -- Harvard Apparatus offers patented 96-Well DispoEquilibrium DIALYZERSTM that are FDA listed and Compliant for In-Vitro diagnostic protocols.

The 96-Well DispoEquilibrium DIALYZER™ provide simple and reliable technique to analyze up to 96 equilibrium dialysis chambers simultaneously on disposable dialysis plates.

Available only from Harvard Apparatus, these unique ready to use equilibrium dialysis plates are convenient and cost effective tools for ligand binding experiments including serum protein binding, protein-drug binding, protein-protein binding and protein-DNA binding assays.

Equilibrium Dialysis is recognized as the Gold Standard1,2 or the most preferred technique for molecular binding studies as it allows for the direct assays of molecular interactions at close to physiological conditions with minimal effects on equilibrium parameters.

Equilibrium Dialysis has also shown itself to be a valuable technique for sample preparation before candidate analysis by mass spectrometry. Studies have shown enhanced target identification when equilibrium dialysis is used.

Each test well in the plate consists of two chambers separated by a regenerated cellulose membrane with a molecular weight cut off of either 5,000 or 10,000 Daltons . Each chamber holds up to 300µl of sample or buffer.

To decrease the dialysis time, the plate needs to be rotated through 360° in a vertical position. Harvard Apparatus can also supply a 1 or 2 plate rotator or an eight plate rotator with temperature control.

The 96–well plate was designed with SBS footprint and well spacing to meet automation needs.

Other equilibrium dialysis products from Harvard Apparatus include the single-sample DispoEquilibrium dialyzer for volumes up to 100µl or the re-usable PTFE Fast Micro-Equilibrium dialyzer for samples from 25µl to 1500µl and the Multi-Equilibrium Dialysis System for up to 20 samples with volumes from 200µl to 5ml.

1 Cleveland Clinic Foundation Intensive Review of Internal Medicine Med, (1994), 96 pg 531-535 Slag M.F. et al

2 Drug-like Properties Concepts, Structure Design and Methods, (2008) pg372 Edward Kerns

For more information please go to www.harvardapparatus.com or contact the Harvard Apparatus Technical Support Department at techsupport@harvardapparatus.com or call (800)272-2775 to discuss your application.

Harvard Apparatus

84 October Hill Road

Holliston, Massachusetts 01746 USA

Toll Free: 800-272-2775

Phone: 508-893-8999

Fax: 508-429-5732

E-mail: bioscience@harvardapparatus.com

Web: www.harvardapparatus.com

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